Study on Germination Characteristics and Dormancy Breaking Methods of Tilia amurensis Seeds

doi:10.11924/j.issn.1000-6850.casb2021-1015

Abstract

Abstract:

In order to find out the cause of Tilia amurensis seed dormancy and a method to quickly relieve the dormancy, the seed coat permeability, seed germination characteristics and dormancy release methods of Tilia amurensis pure seeds were studied. The permeability test results showed that: when treated with concentrated H₂SO₄, the permeability and water absorption of Tilia amurensis seeds increased significantly. The experiment of seed germination characteristics showed that the complete seed germination rate of Tilia amurensis was 0. After removing the seed coat, the germination rate of seeds with intact endosperm, partially removed endosperm (root tip was not exposed), cap-stripped endosperm with exposed root tip, and in vitro embryos was 4.0%, 23.3%, 84.7%, and 100%, respectively. The results show that Tilia amurensis seeds have dormancy, but their seed embryos do not have dormancy. The dormancy is caused by extraembryonic factors, and endosperm (especially endosperm cap) is the main cause of seed dormancy of Tilia amurensis. The test results of seed dormancy breaking method show that the dormancy of Tilia amurensis seeds can be quickly relieved by being treated with concentrated H₂SO₄ for 15 min + soaking in 500 mg/L GA₃solution for 12 h + at low temperature stratification for 30 d, reaching a germination rate of 92.0%. In conclusion, the seed coat of Tilia amurensis seeds has a certain permeability barrier, but the endosperm cap is the main reason for its dormancy. The comprehensive treatment method of concentrated H₂SO₄ treatment + GA₃ soaking + low temperature stratification can relieve the dormancy of Tilia amurensis seeds within 30 days.

Key words: Tilia amurensis, dormancy, germination characteristics, dormancy breaking, low temperature stratification

CLC Number:

S723.1

WANG Yinhua, KONG Yuguang, LI Qinghua, WU Dejun, YAN Liping, XU Tao, LU Yizeng, ZHAI Guofeng. Study on Germination Characteristics and Dormancy Breaking Methods of Tilia amurensis Seeds[J]. Chinese Agricultural Science Bulletin, 2022, 38(29): 80-85.

Figures/Tables 12

References 32

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编号	剥除部位
A	无（完整种子）
B	种皮
C	种皮和部分胚乳（根尖未露出）
D	种皮和胚乳帽（露出根尖）
E	种皮和全部胚乳

编号	剥除部位
A	无（完整种子）
B	种皮
C	种皮和部分胚乳（根尖未露出）
D	种皮和胚乳帽（露出根尖）
E	种皮和全部胚乳

水平	浓硫酸处理时间/min	发芽率/%	GA₃浓度/(mg/L)	发芽率/%
1	5	11.3 b	200	2.3 a
2	10	12.3 b	500	3.3 a
3	15	14.3 a	1000	3.3 a

水平	浓硫酸处理时间/min	发芽率/%	GA₃浓度/(mg/L)	发芽率/%
1	5	11.3 b	200	2.3 a
2	10	12.3 b	500	3.3 a
3	15	14.3 a	1000	3.3 a

浓H₂SO₄处理时间/min	发芽率/%	GA₃浓度/(mg/L)	发芽率/%
15	88.3 a	500	87.7 a
10	84.7 b	200	85.3 a
5	80.7 c	800	80.7 b