SA Priming of Maize Seeds at Different Temperature Under Drought Stress: Effects on Seed Germination and Seedling Physiological Characteristics

doi:10.11924/j.issn.1000-6850.casb2020-0504

Abstract

Abstract:

The aim is to investigate the effect of SA priming at different temperature on maize seed germination, growth and physiological characteristics of maize seedling under drought stress. The maize varieties ‘Zhengdan 958’, ‘Jingtian 808’ and ‘Jingkenuo 2000’ were used as materials, and five treatments including CK, 5, 10, 15 and 20℃ were designed to study the seedling growth performance under drought stress. The results showed that SA priming significantly enhanced the antioxidant enzyme activities, increased the content of osmotic regulatory substances, and decreased the content of MDA in maize seedling, alleviated the effects of drought stress on the growth of maize. Among them, the maize germination parameters (germination rate, germination potential, germination index and vigor index) of ‘Zhengdan 958’ and ‘Jingtian 808’ were the highest at 15℃ priming treatment, increased by 16.67%, 21.26%, 17.69%, 25.39% and 10.88%, 8.76%, 10.02%, 26.83%, respectively, compared with those of the control; while for ‘Jingkenuo 2000’, the priming temperature of 10℃, could achieve an increase of 16.48%, 10.73%, 14.27% and 32.00% compared with those of the control. In conclusion, seed priming can enhance the germination ability of maize seeds under drought stress, and the study could provide a theoretical basis for the application of seed priming to maize production in dryland.

Key words: maize, seed priming, salicylic acid, temperature, dehydration stress, drought stress

CLC Number:

S513

Hou Linxin, Lv Qiang, Huang Ming, Jiao Nianyuan, Yin Fei, Liu Ling, Lv Meng, Fu Guozhan. SA Priming of Maize Seeds at Different Temperature Under Drought Stress: Effects on Seed Germination and Seedling Physiological Characteristics[J]. Chinese Agricultural Science Bulletin, 2021, 37(19): 13-22.

Figures/Tables 8

References 45

[1]	李少昆, 赵久然, 董树亭, 等. 中国玉米栽培研究进展与展望[J]. 中国农业科学, 2017, 50(11):1941-1959.
[2]	王崇桃, 李少昆. 玉米生产限制因素评估与技术优先序[J]. 中国农业科学, 2010, 43(6):1136-1146.
[3]	山仑. 中国西北地区植物水分研究与旱地农业增产[J]. 植物生理学通讯, 1983(5):7-10.
[4]	田锦芬. 干旱对玉米生长发育的影响及预防措施[J]. 北京农业, 2013(21):39.
[5]	Heydecker W. Germination of an idea: the priming of seeds[M]. Nottingham: University of Nottingham School of Agriculture Report, 1973:50-67.
[6]	李振华, 王建华. 种子活力与萌发的生理与分子机制研究进展[J]. 中国农业科学, 2015, 48(4):646-660.
[7]	Ali Lawan Gana, Nulit Rosimah, Ibrahim Mohd Hafiz, et al. Enhancement of germination and early seedling growth of rice (Oryza sativa) var. FARO44 by seed priming under normal and drought stressed conditions[J]. Journal of Plant Nutrition, 2020, 43(11):1579-1593. doi: 10.1080/01904167.2020.1739298 URL
[8]	Nedunchezhiyan, Velusamy, Subburamu. Seed priming to mitigate the impact of elevated carbon dioxide associated temperature stress on germination in rice (Oryza sativa L.)[J]. Archives of Agronomy and Soil Science, 2020, 66(1):83-95. doi: 10.1080/03650340.2019.1599864
[9]	Hussain Sajid, Zhu Chunquan, Huang Jie, et al. Ethylene response of salt stressed rice seedlings following Ethephon and 1-methylcyclopropene seed priming[J]. Plant Growth Regulation, 2020(1):1-13.
[10]	Tabassum Tahira, Farooq Muhammad, Ahmad Riaz, et al. Terminal drought and seed priming improves drought tolerance in wheat[J]. Physiology and Molecular Biology of Plants, 2018, 24(5):845-856. doi: 10.1007/s12298-018-0547-y pmid: 30150859
[11]	Shahid Farooq, Mubshar Hussain, Khawar Jabran, et al. Osmopriming with CaCl₂ improves wheat (Triticum aestivum L.) production under water-limited environments[J]. Environmental science and pollution research international, 2017, 24(15):13638-13649. doi: 10.1007/s11356-017-8957-x pmid: 28391467
[12]	史雨刚, 孙黛珍, 雷逢进. 种子引发对NaCl胁迫下小麦幼苗生理特性的影响[J]. 核农学报, 2011, 25(2):342-347.
[13]	Zhang Y, Zhang C F, Hu J, et al. Sand priming in relation to physiological changes in seed germination and seedling growth of waxy maize under high-salt stress[J]. Seed Science and Technology, 2007, 35(3):733-738. doi: 10.15258/sst URL
[14]	郑昀晔, 曹栋栋, 张胜. 多胺对玉米种子吸胀期间耐冷性和种子发芽能力的影响[J]. 作物学报, 2008, 34(2):261-267.
[15]	李洁, 徐军桂, 林程, 等. 引发对低温胁迫下不同类型玉米种子萌发及幼苗生理特性的影响[J]. 植物生理学报, 2016, 52(2):157-166.
[16]	杜锦, 肖萌, 郝娜娜, 等. 不同药剂引发对干旱胁迫下玉米种子萌发及幼苗生长的影响[J]. 种子, 2014, 33(11):43-46.
[17]	贺红娟, 管桦, 张存莉. 水引发对油松种子和幼苗的生理效应及其作用机理[J]. 生态学报, 2015, 35(21):7033-7042.
[18]	Shah R A, Sharma A, Wali V K, et al. Effect of seed priming on peach, plum and apricot germination and subsequent seeding growth[J]. Indian Journal of Horticulture, 2013, 70(4):591-594.
[19]	李秀梅, 古吉, 李亚清. 不同引发温度及时间对烟草种子低温萌发的影响[J]. 种子, 2020, 39(5):99-103.
[20]	王彦荣. 种子引发的研究现状[J]. 草业学报, 2004, 13(4):7-12.
[21]	孟雪娇, 邸昆, 丁国华. 水杨酸在植物体内的生理作用研究进展[J]. 中国农学通报, 2010, 26(15):207-214.
[22]	谭龙涛, 喻春明, 陈平, 等. 外源水杨酸对干旱胁迫及复水下苎麻生理特性和产量的影响[J]. 核农学报, 2016, 30(2):388-395.
[23]	Zhang K G. Salicylic acid changes activities of H₂O₂-metabolizing enzymes and increases the chilling tolerance of banana seedlings[J]. Environmental and Experimental Botany, 2003, 50(1):9-15. doi: 10.1016/S0098-8472(02)00109-0 URL
[24]	Hua S Q. Effects of different treatments of salicylic acid on heat tolerance, chlorophyll fluorescence, and antioxidant enzyme activity in seedlings of Cucumis sativa L.[J]. Plant Growth Regulation, 2006, 48(2):127-135. doi: 10.1007/s10725-005-5482-6 URL
[25]	杨若鹏, 毕红才, 李杰. 水杨酸对黄瓜种子萌发及干旱胁迫下幼苗生长的影响[J]. 北方园艺, 2018(6):23-29.
[26]	高巧红, 丁希政, 牛志浩, 等. 水杨酸引发处理对低温吸胀期间玉米种子生理指标的影响[J]. 河南农业科学, 2017, 46(3):41-46.
[27]	Sharma Vikas, Prasanna Radha, Hossain Firoz, et al. Priming maize seeds with cyanobacteria enhances seed vigour and plant growth in elite maize inbreds[J]. 3 Biotech, 2020, 10(4):1183-1192.
[28]	Sime Getachew, Aune J B. On-farm seed priming and fertilizer micro-dosing: Agronomic and economic responses of maize in semi-arid Ethiopia[J]. Food and Energy Security, 2020, 9(1):854-869.
[29]	Kawatra Misha, Kaur Kamaljit, Kaur Gurjit. Effect of osmo priming on sucrose metabolism in spring maize, during the period of grain filling, under limited irrigation conditions[J]. Physiology and molecular biology of plants: an international journal of functional plant biology, 2019, 25(6):1367-1376. doi: 10.1007/s12298-019-00706-z URL
[30]	Rasool Tassadduq, Ahmad Riaz, Farooq Muhammad. Seed priming with micronutrients for improving the quality and yield of hybrid maize[J]. Gesunde Pflanzen, 2019, 71(1):37-44. doi: 10.1007/s10343-018-00440-8
[31]	孙园园, 孙永健, 王明田, 等. 种子引发对水分胁迫下水稻发芽及幼苗生理性状的影响[J]. 作物学报, 2010, 36(11):1931-1940.
[32]	王晶英, 敖红, 张杰. 植物生理生化实验技术与原理[M]. 哈尔滨: 东北林业大学出版社, 2003.
[33]	李合生. 植物生理生化实验技术[M]. 北京: 高等教育出版社, 2000.
[34]	Griffith O W. Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine[J]. Anal biochem, 1980, 106(1):207-212. pmid: 7416462
[35]	郭瑞盼, 辛泽毓, 王志强, 等. 干旱胁迫对小麦非结构性碳水化合物代谢的影响及其与抗旱性的关系[J]. 华北农学报, 2015, 30(2):202-211.
[36]	张彩芳. 水杨酸和沙引发对糯玉米种子在盐逆境下发芽及生理特性的影响[D]. 杭州: 浙江大学, 2007.
[37]	何莉芳, 郭世荣, 阎君, 等. 不同处理对芹菜种子萌发过程中抗氧化系统及激素水平的影响[J]. 中国农学通报, 2020, 36(22):31-37.
[38]	刘慧霞. 紫花苜蓿种子水引发研究[D]. 兰州: 兰州大学, 2007:29-36.
[39]	Chiu K Y, Chen C L, Sung J M. Effect of priming temperature on storability of primed sh-2 sweet corn seed[J]. Crop Sci, 2002, 42:1996-2003. doi: 10.2135/cropsci2002.1996 URL
[40]	王飞. 低温锻炼萌动棉种对提高棉花抗冷性的研究[D]. 太谷:山西农业大学, 2013:12-17.
[41]	马超, 孔蓓蓓, 张均, 等. 不同引发剂处理对水分胁迫下小麦发芽及幼苗生理特性的影响[J]. 核农学报, 2017, 31(2):357-363.
[42]	许高平, 刘秀峰, 袁文娅, 等. 水杨酸和甜菜碱浸种对低温干旱胁迫下玉米苗期生长的影响[J]. 玉米科学, 2018, 26(6):50-56.
[43]	李娜, 李志军, 李培环, 等. 不同盐浓度处理对三种结缕草K⁺、Na⁺含量和膜质过氧化的影响[J]. 中国农学通报, 2010, 26(10):152-155.
[44]	吕庆, 郑荣梁. 干旱及活性氧引起小麦膜脂过氧化与脱酯化[J]. 中国科学C辑:生命科学, 1996:26-30.
[45]	Boudmyxay K, 沈镭, 钟帅, 等. 脯氨酸引发提高烟草种子和幼苗抗逆性及其与抗氧化系统的关系[J]. 山西农业科学, 2019, 47(1):39-48.

品种	温度/℃	发芽率/%	发芽势/%	发芽指数	活力指数
郑单958	CK	64.00c	53.33c	6.07d	4.85c
	5	71.33ab	58.67b	6.67bc	5.45bc
	10	72.00ab	65.33a	6.99ab	6.45a
	15	74.67a	64.67a	7.14a	6.80a
	20	67.33b	56.00bc	6.37cd	5.24c
金甜808	CK	61.33b	53.33c	5.89b	3.39b
	5	65.33ab	58.67ab	6.31a	3.88ab
	10	66.67a	60.00a	6.47a	4.12a
	15	68.00a	58.00a	6.47a	4.30a
	20	63.33ab	56.67bc	6.12ab	3.87b
京科糯2000	CK	64.67d	49.97b	6.00c	3.91d
	5	69.33cd	52.67ab	6.37bc	4.32bc
	10	75.33ab	55.33a	6.86a	5.16a
	15	72.00bc	55.33a	6.66ab	4.58bc
	20	68.67cd	52.00ab	6.36bc	4.29cd

品种	温度/℃	发芽率/%	发芽势/%	发芽指数	活力指数
郑单958	CK	64.00c	53.33c	6.07d	4.85c
	5	71.33ab	58.67b	6.67bc	5.45bc
	10	72.00ab	65.33a	6.99ab	6.45a
	15	74.67a	64.67a	7.14a	6.80a
	20	67.33b	56.00bc	6.37cd	5.24c
金甜808	CK	61.33b	53.33c	5.89b	3.39b
	5	65.33ab	58.67ab	6.31a	3.88ab
	10	66.67a	60.00a	6.47a	4.12a
	15	68.00a	58.00a	6.47a	4.30a
	20	63.33ab	56.67bc	6.12ab	3.87b
京科糯2000	CK	64.67d	49.97b	6.00c	3.91d
	5	69.33cd	52.67ab	6.37bc	4.32bc
	10	75.33ab	55.33a	6.86a	5.16a
	15	72.00bc	55.33a	6.66ab	4.58bc
	20	68.67cd	52.00ab	6.36bc	4.29cd

品种	温度/℃	株高/cm	地上鲜重/g	根长/cm	地下鲜重/g	整株鲜重/g
郑单958	CK	6.10b	0.19a	7.50b	0.14b	0.79b
	5	6.18b	0.20a	7.74a	0.15ab	0.82ab
	10	6.46a	0.21a	9.34a	0.15ab	0.92a
	15	6.60a	0.22a	9.47a	0.17a	0.95a
	20	6.18b	0.20a	8.76a	0.15ab	0.82b
金甜808	CK	5.25b	0.13b	6.36de	0.09b	0.58b
	5	5.84ab	0.16ab	7.58cd	0.10ab	0.61ab
	10	6.04ab	0.18ab	9.54ab	0.13a	0.63ab
	15	6.06a	0.19a	11.00a	0.12ab	0.67a
	20	5.85ab	0.16ab	8.12bc	0.11b	0.63ab
京科糯2000	CK	4.98b	0.11b	7.73b	0.11b	0.65b
	5	5.94b	0.12ab	8.18ab	0.12ab	0.68b
	10	6.92a	0.15a	9.66a	0.13a	0.75a
	15	5.48b	0.13ab	9.08a	0.11ab	0.69ab
	20	5.11b	0.12ab	8.10b	0.12ab	0.68ab

品种	温度/℃	株高/cm	地上鲜重/g	根长/cm	地下鲜重/g	整株鲜重/g
郑单958	CK	6.10b	0.19a	7.50b	0.14b	0.79b
	5	6.18b	0.20a	7.74a	0.15ab	0.82ab
	10	6.46a	0.21a	9.34a	0.15ab	0.92a
	15	6.60a	0.22a	9.47a	0.17a	0.95a
	20	6.18b	0.20a	8.76a	0.15ab	0.82b
金甜808	CK	5.25b	0.13b	6.36de	0.09b	0.58b
	5	5.84ab	0.16ab	7.58cd	0.10ab	0.61ab
	10	6.04ab	0.18ab	9.54ab	0.13a	0.63ab
	15	6.06a	0.19a	11.00a	0.12ab	0.67a
	20	5.85ab	0.16ab	8.12bc	0.11b	0.63ab
京科糯2000	CK	4.98b	0.11b	7.73b	0.11b	0.65b
	5	5.94b	0.12ab	8.18ab	0.12ab	0.68b
	10	6.92a	0.15a	9.66a	0.13a	0.75a
	15	5.48b	0.13ab	9.08a	0.11ab	0.69ab
	20	5.11b	0.12ab	8.10b	0.12ab	0.68ab

指标	主成分1	主成分2	主成分3	主成分4	主成分5	主成分6	主成分7	主成分8	主成分9
发芽率	0.780	0.532	-0.303	-0.028	-0.077	-0.093	0.048	0.014	0.004
发芽势	0.810	-0.124	0.360	-0.441	-0.035	0.035	-0.046	0.009	0.001
发芽指数	0.905	0.360	-0.085	-0.198	-0.043	-0.045	0.006	-0.017	-0.008
活力指数	0.946	-0.100	-0.281	-0.037	0.068	0.098	0.011	-0.011	0.011
株高	0.837	0.000	0.193	0.258	-0.438	0.048	-0.023	0.000	0.000
地上重	0.802	-0.491	0.297	0.054	0.052	-0.087	0.119	-0.002	0.000
根长	0.617	0.544	0.446	0.214	0.275	0.055	-0.005	0.001	0.000
地下重	0.914	-0.288	-0.104	0.155	0.132	-0.128	-0.116	0.000	0.001
整株鲜重	0.894	-0.249	-0.328	0.067	0.109	0.118	0.018	0.010	-0.009
特征值	6.338	1.119	0.751	0.380	0.314	0.064	0.033	0.001	0.000
贡献率	70.423	12.437	8.341	4.220	3.484	0.716	0.367	0.009	0.003