Abstract:

This study aims at revealing the changes of bacterial community in the covering soil of Ganoderma lingzhi with different continuous cropping years, and providing a theoretical basis for the study of continuous cropping obstacle mechanism of Ganoderma lingzhi. Taking the adjacent sandy soil (GL0) as the control, Illumina MiSeq platform was used for the amplicon sequencing of the covering soil of Ganoderma lingzhi cultivated for 1 year (GL1), and continuous cropped for 1 year (GL2), 2 years (GL3), and 3 years (GL4). The diversity analysis showed that continuous cropping increased the difference in species composition of bacterial communities in the covering soil of Ganoderma lingzhi, and the abundance and diversity of bacterial communities showed a tendency of increasing first and then decreasing. At the level of phylum, the relative abundance of Proteobacteria and Actinomycetes increased significantly with the increase of continuous cropping years, while that of Chloroflex decreased. At the genus level, the relative abundance of Sphingomonas, Anaeromyxobacter, Bradyrhizobium, Dehalococcoides and Ktedonobacter in the covering soil of Ganoderma lingzhi reduced year by year; while that of Lysobacter, Gp6 and Gp16 dramatically increased. Continuous cropping obstacle of Ganoderma lingzhi may be related to the year-by-year decrease of the relative abundance of beneficial bacteria such as Sphingomonas, Anaeromyxobacter, Bradyrhizobium and Dehalococcoides and so on in covering soil.

Key words: Ganoderma lingzhi, bacterial communities, bacterial diversity, amplicon sequencing, continuous cropping obstacle