Determination of Five Kinds of Flavonoids in the Medicinal Herbs of Thesium chinensis

doi:10.11924/j.issn.1000-6850.casb2021-0737

Abstract

Abstract:

To reveal the differences in the contents of five kinds of flavonoids between wild and cultivated Thesium chinensis, the contents of Kaempferol-3-O-rhamnose(1-2)glucoside, Kaempferol -3-O- rutinoside, Astragaline, Kaempferol-7-glucoside and Apigenin-7-O-β-D-glucopyranoside in Thesium chinensis were determined by Ultra-High Performance Liquid Phase. The results showed that the linear ranges of Kaempferol-3-O-rhamnose(1-2)glucoside, Kaempferol -3-O- rutinoside, Astragaline, Kaempferol-7-glucoside and Apigenin-7-O-β-D-glucopyranoside were 10-1000 (0.9995), 1-200 (0.9992), 1-200 (0.9995), 1-200 (0.9994), 10-2000 g/mL (0.9998), respectively; RSD of precision, stability and reproducibility tests was 0.17%~3%. The average recoveries of the five kinds of flavonoids were 91.99%~107.40% with RSD less than 3.24%. Compared with that of the wild Thesium chinensis, the contents of Kaempferol-3-O-rhamnose(1-2)glucoside, Astragaline, Kaempferol-7-glucoside and Apigenin-7-O-β-D-glucopyranoside in cultivated Thesium chinensis were significantly higher (P<0.05), and the content of Kaempferol -3-O- rutinoside tended to be higher but showed no significant difference. This study shows that the method has good repeatability, accuracy and simplicity. It can determine the contents of the five kinds of flavonoids in wild and cultivated Thesium chinensis at the same time, and is recommended to be popularized.

Key words: Thesium chinensis, flavonoids chemical constituents, Ultra-High Performance Liquid Phase, wild and cultivated, orthogonal test

CLC Number:

S567.23

ZHENG Lingling, JI Ruifeng, HAN Hongliang, LUO Yilu, LUO Jiayi, GUAN Yueqin, YAO Qisheng, ZHENG Weibing, CHEN Meilan, ZHOU Xiuteng. Determination of Five Kinds of Flavonoids in the Medicinal Herbs of Thesium chinensis[J]. Chinese Agricultural Science Bulletin, 2022, 38(5): 30-36.

Figures/Tables 10

References 25

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水平	A（料液比）	B（时间/min）	C（溶剂比例/%）
1	1：10	30	40
2	1：20	45	60
3	1：30	60	80

水平	A（料液比）	B（时间/min）	C（溶剂比例/%）
1	1：10	30	40
2	1：20	45	60
3	1：30	60	80

处理	A	B	C	总黄酮类含量/(mg/g)
1	1	1	1	54.47
2	1	2	2	57.63
3	1	3	3	55.44
4	2	1	2	30.95
5	2	2	3	30.22
6	2	3	1	30.55
7	3	1	3	12.61
8	3	2	1	12.20
9	3	3	2	13.52
K1	167.54	98.03	97.23	-
K2	91.72	100.04	102.10	-
K3	38.33	99.52	98.26	-
k1	55.85	32.68	32.41	-
k2	30.57	33.35	34.03	-
k3	12.78	33.17	32.75	-
极差R	43.07	0.49	1.62	-
主次顺序	A>C>B			-
优水平	A₁	B₂	C₂	-
优组合	A₁B₂C₂			-

处理	A	B	C	总黄酮类含量/(mg/g)
1	1	1	1	54.47
2	1	2	2	57.63
3	1	3	3	55.44
4	2	1	2	30.95
5	2	2	3	30.22
6	2	3	1	30.55
7	3	1	3	12.61
8	3	2	1	12.20
9	3	3	2	13.52
K1	167.54	98.03	97.23	-
K2	91.72	100.04	102.10	-
K3	38.33	99.52	98.26	-
k1	55.85	32.68	32.41	-
k2	30.57	33.35	34.03	-
k3	12.78	33.17	32.75	-
极差R	43.07	0.49	1.62	-
主次顺序	A>C>B			-
优水平	A₁	B₂	C₂	-
优组合	A₁B₂C₂			-

百蕊草化学成分	回归方程	线性范围/(µg/mL)	回归系数r
百蕊草素Ⅰ	y=1148.9x+14942	10-1000	0.9995
山奈酚-3-O-芸香糖苷	y=4539.8x+7932.5	1-200	0.9992
紫云英苷	y=5664x-18270	1-200	0.9995
山奈酚-7-O-葡萄糖苷	y=5881.1x-21385	1-200	0.9994
芹菜素-7-O-β-D-吡喃葡萄糖苷	y=4446x+28772	10-2000	0.9998