Control Effect of Three Bacillus subtilis Strains on Tobacco Powdery Mildew

doi:10.11924/j.issn.1000-6850.casb2021-0817

Abstract

Abstract:

To clarify the control effects of three Bacillus subtilis strains on powdery mildew of flue-cured tobacco variety ‘Yunyan 87’, suspensions, bacterial suspension, and suspension agent (SC) prepared by B. subtilis and low-toxic chemical pesticides were used as test materials to carry out research on their control effects on powdery mildew. The indoor results showed that: YWF-19-0068, YWF-19-0070 and YWF-19-0071 biocontrol agents of 10 times dilution had the control effect of 70.49%, 61.15% and 69.71%, respectively. And 50 times dilution of bacterial suspension agents had the germination inhibition rate on the powdery mildew spore of 56.92%, 56.92% and 58.46%, respectively. The outdoor results showed that: 10 days after the application of the three biocontrol agents at different dosages, the control effects of 50 times dilution of bacterial suspension agents were the best of 46.85%, 65.50%, and 51.70%, respectively. After full and half doses of thiophanate were used once, YWF-19-0070 (SC) was applied once every 3 days. 10 days after the last application, the control effect of the full dosage thiophanate methyl treatment was more than 77.73%, and the control effect of the half dosage thiophanate methyl treatment was more than 60.23%.

Key words: Bacillus subtilis, suspension agents, tobacco, powdery mildew, control effect

CLC Number:

S435.72

CAI Yongzhan, WANG Ruibao, BAI Tao, LIU Youcai, HAN Xiaonv, WANG Bin, HUA Xiaobing, LI Cheng, MAO Minglin, ZI Wenlin. Control Effect of Three Bacillus subtilis Strains on Tobacco Powdery Mildew[J]. Chinese Agricultural Science Bulletin, 2022, 38(25): 125-129.

Figures/Tables 4

References 29

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处理	使用浓度	病情指数	防效/%
YWF-19-0068	1.0×10⁸CFU/mL	11.56±1.31c	70.49±1.25a
YWF-19-0070	1.0×10⁸CFU/mL	23.02±2.04b	61.15±1.83bc
YWF-19-0071	1.0×10⁸CFU/mL	17.95±1.23b	69.71±1.5ab
25%嘧菌酯SC	125 mg/L	12.62±1.78c	78.70±3.1a
清水		59.26±1.52a

处理	使用浓度	病情指数	防效/%
YWF-19-0068	1.0×10⁸CFU/mL	11.56±1.31c	70.49±1.25a
YWF-19-0070	1.0×10⁸CFU/mL	23.02±2.04b	61.15±1.83bc
YWF-19-0071	1.0×10⁸CFU/mL	17.95±1.23b	69.71±1.5ab
25%嘧菌酯SC	125 mg/L	12.62±1.78c	78.70±3.1a
清水		59.26±1.52a

菌株	稀释倍数	萌发孢子平均数/个	镜检孢子总数/个	萌发率/%	抑制率/%
YWF-19-0068	50倍	14.0	150	9.33	56.92
	100倍	19.0	150	12.67	41.54
	150倍	22.5	150	15.00	30.77
YWF-19-0070	50倍	14.0	150	9.33	56.92
	100倍	21.0	150	14.00	35.38
	150倍	24.5	150	16.33	24.62
YWF-19-0071	50倍	13.5	150	9.00	58.46
	100倍	21.5	150	14.33	33.85
	150倍	25.0	150	16.67	23.08
CK		32.5	150	21.67

菌株	稀释倍数	萌发孢子平均数/个	镜检孢子总数/个	萌发率/%	抑制率/%
YWF-19-0068	50倍	14.0	150	9.33	56.92
	100倍	19.0	150	12.67	41.54
	150倍	22.5	150	15.00	30.77
YWF-19-0070	50倍	14.0	150	9.33	56.92
	100倍	21.0	150	14.00	35.38
	150倍	24.5	150	16.33	24.62
YWF-19-0071	50倍	13.5	150	9.00	58.46
	100倍	21.5	150	14.33	33.85
	150倍	25.0	150	16.67	23.08
CK		32.5	150	21.67

菌株	稀释倍数	药前病情指数	药后3 d		药后7 d		药后10 d
菌株	稀释倍数	药前病情指数	病情指数	防效/%	病情指数	防效/%	病情指数	防效/%
YWF-19-0068	50倍液	5.77±0.10e	4.19±0.12g	43.93±1.76a	4.80±0.13f	54.10±1.97d	5.42±0.28de	46.85±3.14cd
	100倍液	7.15±0.10c	6.83±0.20c	26.97±0.27d	7.75±0.08c	43.72±1.29f	7.82±0.58c	43.48±1.97de
	150倍液	8.73±0.06a	8.26±0.16a	26.20±1.11d	8.90±0.46b	40.13±0.89g	8.72±0.50b	38.02±1.60f
YWF-19-0070	50倍液	6.38±0.20d	5.04±0.14e	39.05±2.12b	3.94±0.29g	65.98±1.06a	3.89±0.25f	65.50±2.37a
	100倍液	5.98±0.12e	5.42±0.11d	30.22±1.17c	4.12±0.47g	62.04±1.34b	3.67±0.36f	65.23±1.84a
	150倍液	7.33±0.19c	6.63±0.19c	30.08±0.95c	5.80±0.26e	56.34±1.55cd	6.12±0.65d	52.79±5.43b
YWF-19-0071	50倍液	4.61±0.10h	4.60±0.11f	39.21±2.30b	5.00±0.59f	57.05±1.67c	4.90±0.33e	51.70±1.60b
	100倍液	4.86±0.09g	5.31±0.13de	23.02±3.28e	5.12±0.16f	41.88±2.06fg	5.48±0.64de	39.83±0.64ef
	150倍液	8.42±0.08b	6.63±0.11c	15.68±1.85g	6.55±0.46d	40.18±0.22g	7.18±0.33c	36.09±1.01f
清水对照		5.54±0.13f	7.18±0.09b		10.05±0.43a		9.79±0.52a
甲基硫菌灵（全量）		6.41±0.09d	6.67±0.31c	19.64±1.28f	5.83±0.17e	49.83±1.22e	5.64±0.62de	50.15±1.78bc