Shading-Tolerant Spring Soybean: Genotypes Screening and Identification of Shade Tolerance Indexes

doi:10.11924/j.issn.1000-6850.casb2022-0821

Abstract

Abstract:

In order to study the effect of shading stress on spring soybean in the Huanghuaihai region, and to screen the identification indexes and mathematical evaluation model of spring soybean shade tolerance, the shade tolerance coefficients of 10 indicators of soybean were used to measure the shade tolerance of individual indicators, and the comprehensive shade tolerance evaluation value of 25 soybean genotypes was calculated by principal component analysis, membership function method and stepwise regression method. The results showed that different soybean genotypes responded differently to shading stress. The plant height, the node of main stem and average internode length increased to different degrees under shade conditions, and the degree of variation increased, while the pods per plant, grains per plant, grain-stem ratio, grain weight per plant and grain per pod decreased significantly. Based on the cluster analysis of shade tolerance evaluation value (D), the 25 test soybean genotypes were divided into five categories: strong shade-tolerant, more shade-tolerant, moderate shade-tolerant, shade-intolerant and extremely shade tolerant. A mathematical model for the evaluation of shade tolerance of spring soybeans in the Huanghuaihai region was established: D=-0.149 + 0.202X₀₁-0.054X₀₄+ 0.233X₀₅+ 0.233X₀₇ (R²=0.963), and the prediction accuracy reached 82%. In summary, four indexes, including the plant height, the height of lower pods, the average internode length and grains per plant, were screened as the identification indexes of Huanghuaihai shade-tolerant spring soybean, and six strong shade-tolerant soybean materials were identified.

Key words: soybean, shading stress, shade tolerance evaluation, principal components analysis, membership function method, comprehensive evaluation

ZHAO Zhixin, FU Mengmeng, LI Shuguang, WANG Yaqi, YU Xiwen, ZHANG Hongmei, CHEN Huatao, XU Haifeng. Shading-Tolerant Spring Soybean: Genotypes Screening and Identification of Shade Tolerance Indexes[J]. Chinese Agricultural Science Bulletin, 2023, 39(33): 25-32.

Figures/Tables 8

References 24

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编号	品系	编号	品系	编号	品系	编号	品系	编号	品系
M01	JB20-2030	M06	JB20-4134	M11	中黄13	M16	M19-142	M21	邯617
M02	JB20-2066	M07	JB20-5131	M12	M21-91(0)	M17	M19-155	M22	阜豆1610
M03	JB20-3119	M08	JB20-6138	M13	M17-95-1	M18	中豆5701	M23	驻豆29
M04	JB20-4109	M09	JB20-6142	M14	M18-39-6	M19	南农1138-2	M24	徐豆12
M05	JB20-4124	M10	HJ-183	M15	M18-229	M20	科丰14	M25	灌云11-68

编号	品系	编号	品系	编号	品系	编号	品系	编号	品系
M01	JB20-2030	M06	JB20-4134	M11	中黄13	M16	M19-142	M21	邯617
M02	JB20-2066	M07	JB20-5131	M12	M21-91(0)	M17	M19-155	M22	阜豆1610
M03	JB20-3119	M08	JB20-6138	M13	M17-95-1	M18	中豆5701	M23	驻豆29
M04	JB20-4109	M09	JB20-6142	M14	M18-39-6	M19	南农1138-2	M24	徐豆12
M05	JB20-4124	M10	HJ-183	M15	M18-229	M20	科丰14	M25	灌云11-68

编号	X₀₁	X₀₂	X₀₃	X₀₄	X₀₅	X₀₆	X₀₇	X₀₈	X₀₉	X₁₀
M01	1.74	1.41	1.00	1.56	1.23	0.74	0.77	0.44	0.56	1.04
M02	1.01	0.98	0.20	2.69	1.04	0.63	0.62	0.61	0.64	0.98
M03	0.99	0.96	0.88	0.45	1.03	0.58	0.64	0.64	0.61	1.09
M04	1.06	1.10	0.36	2.17	0.97	0.81	0.47	0.44	0.44	0.58
M05	1.15	1.05	1.33	0.68	0.83	0.88	0.76	0.66	0.68	0.87
M06	1.51	1.00	1.50	0.94	1.51	0.56	0.69	0.46	0.62	1.24
M07	1.20	0.93	0.67	0.44	0.90	0.61	0.57	0.48	0.48	0.94
M08	1.17	1.09	0.80	1.70	1.08	0.68	0.68	0.57	0.71	1.00
M09	0.92	0.93	0.80	0.51	1.00	0.84	0.90	0.97	0.77	1.08
M10	1.05	1.10	0.34	1.09	0.95	0.84	0.73	0.70	0.72	0.87
M11	1.00	0.83	1.43	1.08	1.20	1.14	0.78	0.78	0.77	0.68
M12	1.03	1.00	1.33	0.85	1.03	0.76	0.61	0.59	0.70	0.80
M13	0.77	0.98	2.33	0.81	0.79	0.54	0.33	0.43	0.33	0.61
M14	0.64	0.67	0.78	0.68	0.96	0.47	0.15	0.24	0.70	0.32
M15	1.18	1.00	0.38	0.72	1.18	0.73	0.58	0.49	0.53	0.80
M16	1.07	0.89	1.00	1.06	1.21	0.81	0.76	0.71	0.79	0.95
M17	0.90	0.98	1.29	0.85	0.92	1.01	0.37	0.41	0.82	0.37
M18	1.62	1.21	0.93	0.94	1.34	0.86	0.81	0.50	0.93	0.94
M19	1.34	1.03	0.88	0.88	1.30	0.93	0.78	0.58	0.92	0.84
M20	2.01	1.53	0.80	0.78	1.31	0.72	0.47	0.23	0.39	0.65
M21	1.53	1.33	0.53	1.48	1.15	0.92	0.64	0.42	0.42	0.69
M22	1.00	1.18	0.64	1.72	0.85	0.94	0.76	0.76	0.63	0.80
M23	1.86	0.75	2.60	0.44	1.10	0.91	0.45	0.76	0.40	1.54
M24	1.07	1.13	1.88	0.68	0.95	0.64	0.43	0.41	0.80	0.68
M25	1.01	0.90	0.76	0.41	1.13	1.04	0.99	0.98	0.77	0.95
平均值	1.19	1.04	1.02	1.02	1.08	0.78	0.63	0.57	0.65	0.85
标准差	0.33	0.19	0.58	0.56	0.17	0.17	0.19	0.19	0.16	0.25
变异系数/%	27.82	18.08	57.03	54.65	16.13	21.22	29.88	32.88	25.24	29.90

编号	X₀₁	X₀₂	X₀₃	X₀₄	X₀₅	X₀₆	X₀₇	X₀₈	X₀₉	X₁₀
M01	1.74	1.41	1.00	1.56	1.23	0.74	0.77	0.44	0.56	1.04
M02	1.01	0.98	0.20	2.69	1.04	0.63	0.62	0.61	0.64	0.98
M03	0.99	0.96	0.88	0.45	1.03	0.58	0.64	0.64	0.61	1.09
M04	1.06	1.10	0.36	2.17	0.97	0.81	0.47	0.44	0.44	0.58
M05	1.15	1.05	1.33	0.68	0.83	0.88	0.76	0.66	0.68	0.87
M06	1.51	1.00	1.50	0.94	1.51	0.56	0.69	0.46	0.62	1.24
M07	1.20	0.93	0.67	0.44	0.90	0.61	0.57	0.48	0.48	0.94
M08	1.17	1.09	0.80	1.70	1.08	0.68	0.68	0.57	0.71	1.00
M09	0.92	0.93	0.80	0.51	1.00	0.84	0.90	0.97	0.77	1.08
M10	1.05	1.10	0.34	1.09	0.95	0.84	0.73	0.70	0.72	0.87
M11	1.00	0.83	1.43	1.08	1.20	1.14	0.78	0.78	0.77	0.68
M12	1.03	1.00	1.33	0.85	1.03	0.76	0.61	0.59	0.70	0.80
M13	0.77	0.98	2.33	0.81	0.79	0.54	0.33	0.43	0.33	0.61
M14	0.64	0.67	0.78	0.68	0.96	0.47	0.15	0.24	0.70	0.32
M15	1.18	1.00	0.38	0.72	1.18	0.73	0.58	0.49	0.53	0.80
M16	1.07	0.89	1.00	1.06	1.21	0.81	0.76	0.71	0.79	0.95
M17	0.90	0.98	1.29	0.85	0.92	1.01	0.37	0.41	0.82	0.37
M18	1.62	1.21	0.93	0.94	1.34	0.86	0.81	0.50	0.93	0.94
M19	1.34	1.03	0.88	0.88	1.30	0.93	0.78	0.58	0.92	0.84
M20	2.01	1.53	0.80	0.78	1.31	0.72	0.47	0.23	0.39	0.65
M21	1.53	1.33	0.53	1.48	1.15	0.92	0.64	0.42	0.42	0.69
M22	1.00	1.18	0.64	1.72	0.85	0.94	0.76	0.76	0.63	0.80
M23	1.86	0.75	2.60	0.44	1.10	0.91	0.45	0.76	0.40	1.54
M24	1.07	1.13	1.88	0.68	0.95	0.64	0.43	0.41	0.80	0.68
M25	1.01	0.90	0.76	0.41	1.13	1.04	0.99	0.98	0.77	0.95
平均值	1.19	1.04	1.02	1.02	1.08	0.78	0.63	0.57	0.65	0.85
标准差	0.33	0.19	0.58	0.56	0.17	0.17	0.19	0.19	0.16	0.25
变异系数/%	27.82	18.08	57.03	54.65	16.13	21.22	29.88	32.88	25.24	29.90

	X₀₁	X₀₂	X₀₃	X₀₄	X₀₅	X₀₆	X₀₇	X₀₈	X₀₉	X₁₀
X₀₁	1
X₀₂	0.581^**	1
X₀₃	0.125	-0.268	1
X₀₄	-0.015	0.322	-0.446^**	1
X₀₅	0.640^**	0.244	-0.065	-0.004	1
X₀₆	0.116	0.038	-0.019	-0.001	0.090	1
X₀₇	0.150	0.164	-0.296	0.043	0.341	0.518^**	1
X₀₈	-0.216	-0.375	0.002	-0.117	-0.093	0.543^**	0.728^**	1
X₀₉	-0.289	-0.13	-0.174	-0.107	0.174	0.332	0.431^*	0.328	1
X₁₀	0.463^*	-0.097	0.211	-0.104	0.346	0.001	0.492^*	0.519^**	-0.065	1