Exopolysaccharide Production by Pediococcus acidilactici J1: Fermentation Conditions Optimization

doi:10.11924/j.issn.1000-6850.casb2023-0247

Abstract

Abstract:

Lactic acid bacteria (LAB) can synthesize exopolysaccharides (EPS) with various functional characteristics, which can improve the flavor of dairy products and have functions such as anticancer, antioxidant, and probiotic properties. However, the low production of LAB EPS limits its industrial production and application. In order to increase the yield of EPS and promote its commercial development, it is very important to optimize the fermentation conditions of strains producing EPS. In this study, the method of single factor test was used to optimize the conditions for EPS production by Pediococcus acidilactici J1. The results showed that under the conditions of 70 g/L sucrose, 14 g/L beef paste, 3.5 g/L ammonium citrate, 4 g/L anhydrous sodium acetate, 4.00% inoculum amount, 35℃ culture temperature, pH 5.5 and 160 r/min shaking speed, EPS yield was the highest, which was (72.66±0.02) g/L. This study significantly improved the EPS yield of P. acidilactici J1, which was 4.76 times of that before optimization. It laid a solid theoretical foundation for the industrial large-scale production of LAB EPS.

Key words: Pediococcus acidilactici, exopolysaccharides, single factor test, fermentation condition, optimization

YU Wenna, YU Liansheng, WANG Shuo, ZHAO Dan, DU Renpeng. Exopolysaccharide Production by Pediococcus acidilactici J1: Fermentation Conditions Optimization[J]. Chinese Agricultural Science Bulletin, 2024, 40(6): 143-151.

Figures/Tables 12

References 34

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	葡萄糖	麦芽糖	果糖	乳糖	蔗糖
细菌数/(CFU/mL)	1.61E+8	7.03E+7	9.85E+7	4.11E+7	7.69E+8
EPS含量/(g/L)	12.65±0.20	2.58±0.12	11.56±0.15	2.50±0.11	15.27±0.14

	葡萄糖	麦芽糖	果糖	乳糖	蔗糖
细菌数/(CFU/mL)	1.61E+8	7.03E+7	9.85E+7	4.11E+7	7.69E+8
EPS含量/(g/L)	12.65±0.20	2.58±0.12	11.56±0.15	2.50±0.11	15.27±0.14

	蛋白胨	酵母浸粉	牛肉膏
细菌数/(CFU/mL)	4.77E+8	4.61E+8	1.17E+8
EPS含量/(g/L)	36.15±2.24	43.76±1.07	45.71±1.18

	蛋白胨	酵母浸粉	牛肉膏
细菌数/(CFU/mL)	4.77E+8	4.61E+8	1.17E+8
EPS含量/(g/L)	36.15±2.24	43.76±1.07	45.71±1.18

	磷酸氢二钾	硫酸镁	无水乙酸钠
细菌数/(CFU/mL)	1.01E+8	1.35E+8	1.34E+8
EPS含量/(g/L)	46.540±0.10	51.908±0.07	55.662±0.20