Analysis of Rhizosphere Bacterial Community Characteristics in the Disease Caused by Root-knot Nematodes on Panax notoginseng

doi:10.11924/j.issn.1000-6850.casb2024-0202

Abstract

Abstract:

Root-knot nematodiasis mainly harms the roots of Panax notoginseng, meanwhile, its compound infection was caused by pathogen invasion of panax notoginseng, which leads to the necrosis and decay of root and ultimately made its yield was decreased. To understand the diversity of bacterial community in rhizosphere soil of Panax notoginseng in both healthy and it was invaded root-knot nematode disease conditions. We collected samples come from healthy soil (HK), infected soil (GB) and blank control (CK), and their bacterial communities were analyzed by using 16S rRNA sequencing. The results showed that the content of saponins (Rg1, Re, Rd) in healthy Panax notoginseng was significantly higher than that in affected by root-knot nematode. In the analysis of community diversity, the number of OTU was 497 in the rhizosphere bacterial community of healthy panax notoginseng, which was significantly higher than that of 427 in susceptible panax notoginseng and of 460 in CK. In the comparison of diversity index (ace, chao1, Shannon), although rhizosphere bacterial community had not significant difference in healthy and root-knot nematode, but it was significantly higher than that of blank control (CK). PCoA analysis showed that the similarity of bacterial community structure was low for three groups treatments (R²=0.544, P=0.008). However, the dominant bacteria existed differences in each treatment soil. Bradyrhizobium, JG30a-KF-32, Saccharimonadales, Granulicella and WPS-2 were significantly enriched in CK. Chujaibacter, Bryobacter, AD3 were significantly enriched in healthy Panax notoginseng. Gemmatimonas and Rhodanobacte were significantly enriched in Panax notoginseng was infected root-knot nematodiasis. The content of saponins was decreased by infecting root-knot nematodes. LEfSe analysis demonstrated that more biocontrol bacteria such as Bryobacter were enriched in the rhizosphere of healthy panax notoginseng, which provided help for the biological control of pathogenic bacteria of panax notoginseng.

Key words: Panax notoginseng, root-knot nematodes, bacterial diversity, 16S rRNA, saponin content, biocontrol bacteria

PENG Cuixian, WANG Can, LI Xueping, QU Yonghan, SUN Hongwei, YANG Yuling, ZHAO Dawei, TAO Yonghong. Analysis of Rhizosphere Bacterial Community Characteristics in the Disease Caused by Root-knot Nematodes on Panax notoginseng[J]. Chinese Agricultural Science Bulletin, 2024, 40(35): 49-55.

Figures/Tables 8

References 22

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Sample	R1	Rg1	Re	Rb1	Rd	Total
GB	0.512±0.210 a	0.669±0.044 b	0.000±0 b	1.001±0.485 a	0.184±0.079 b	2.367±0.817 b
HA	0.404±0.050 a	1.625±0.335 a	0.121±0.209 a	0.948±0.033 a	0.210±0.031 a	3.308±0.568 a

Sample	R1	Rg1	Re	Rb1	Rd	Total
GB	0.512±0.210 a	0.669±0.044 b	0.000±0 b	1.001±0.485 a	0.184±0.079 b	2.367±0.817 b
HA	0.404±0.050 a	1.625±0.335 a	0.121±0.209 a	0.948±0.033 a	0.210±0.031 a	3.308±0.568 a

Sample	#PE_reads	#Nochimera	AvgLen/bp	GC/%
CK1	100898	79171	446.89	55.68
CK2	101228	83975	447.43	55.04
CK3	100025	83953	447.51	55.24
GB1	101406	86569	449.27	55.42
GB2	101085	80107	448.56	55.69
GB3	100412	88387	449.21	55.48
HA1	101492	88061	447.94	55.77
HA2	100741	81642	449.34	55.61
HA3	101718	88279	448.76	55.80

Sample	#PE_reads	#Nochimera	AvgLen/bp	GC/%
CK1	100898	79171	446.89	55.68
CK2	101228	83975	447.43	55.04
CK3	100025	83953	447.51	55.24
GB1	101406	86569	449.27	55.42
GB2	101085	80107	448.56	55.69
GB3	100412	88387	449.21	55.48
HA1	101492	88061	447.94	55.77
HA2	100741	81642	449.34	55.61
HA3	101718	88279	448.76	55.80

Sample	Ace	Chao1	Shannon	Simpson
HA	4138.351±46.009 a	4132.413±65.732 a	9.806±0.060 a	0.997±0.000 a
GB	4213.895±44.190 a	4202.134±51.933 a	9.889±0.052 a	0.997±0.000 a
CK	3821.894±61.765 b	3806.330±71.853 b	9.578±0.149 b	0.996±0.002 a