Identification and Phylogenetic Analysis of Pathogen of Rice Seedling Blight

doi:10.11924/j.issn.1000-6850.casb2024-0296

Abstract

Abstract:

To accurately identify pathogen of rice seedling blight and make its taxonomic status clear, the identification was carried out using morphology examination and net BLAST based on rDNA ITS sequences, the differences on rDNA ITS sequences of the rice seedling blight fungus and its related fungi from the same genus were compared, and the phylogenetic analysis was carried out. The result revealed that the pathogen of rice seedling blight was Fusarium graminearum. The differences of bases were at the sites of 92, 129, 150, 390, 394-397, 399, 401, 403, 405, 408 and 418-419 regions of rDNA ITS sequences on the pathogen of rice seedling blight and F. sporotrichioides, which was its nearer fungi from the same genus. The results of phylogenetic analysis revealed that rice seedling blight fungus clustered with F. graminearumand, and kept a nearer phylogenetic relationship with F. sporotrichioides. Fungi from the 6 genera clustered as 2 groups, and Fusarium had the nearest phylogenetic relationship with Trichoderma.

Key words: rice seedling blight, Fusarium graminearum, rDNA ITS sequences, identification, sequence alignment, phylogenetic analysis

KANG Meihua, DUAN Lingtao, YIN Changfa, QU Runbo, XIAO Sujun, WANG Xi, ZHANG Lu, KUANG Hongmin, SUN Qiang, CHEN Hongfan, YANG Yingqing, SHAO Jianyang, TU Xueqin, LAN Bo. Identification and Phylogenetic Analysis of Pathogen of Rice Seedling Blight[J]. Chinese Agricultural Science Bulletin, 2024, 40(36): 140-146.

Figures/Tables 6

References 27

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