Genome-Wide Identification and Bioinformatics Analysis of DELLAs Gene Family in Longan

doi:10.11924/j.issn.1000-6850.casb2024-0072

Abstract

Abstract:

The DELLA proteins act as the major negative regulatory protein involved in the gibberellin signaling pathway, and they play an important role in plant-environment interactions and the regulation of plant growth and development. In order to explore the structural characteristics and functions of the longan DELLA gene family, this study performed genome-wide identification and bioinformatics analysis of the longan DELLA gene family (named DlDELLA). Based on the 'Jidanben' longan genome and transcriptome data published in our previous study, 10 DlDELLAs were identified, which were distributed on seven different chromosomes and all contained conserved DELLA domains. Subcellular localization analysis revealed that DlDELLAs were located in the nucleus, cytoplasm, chloroplast and endoplasmic reticulum. Phylogenetic tree analysis showed that the 10 DlDELLA proteins were divided into five subfamilies. Cis-acting element analysis showed that the promoter region of DlDELLA genes contained several elements relevant to light response, hormone response, stress response and plant development. The expression analysis revealed significant variations in the expression levels of DlDELLAs among different organs. Most DlDELLA family members were expressed in seeds, roots, stems and flowers, while DlDELLA5 was only expressed in leaves, DlDELLA9 was only expressed in seeds, and DlDELLA10 was only expressed in roots. The above studies enhance our understanding of the DlDELLA gene family in longan and provide theoretical guidance and reference for the study on the function of DlDELLA genes.

Key words: longan, gibberellin, gibberellin signaling pathway, DELLAs gene family, light-responsive elements, gene expression, bioinformatics

WU Kaitong, HUANG Shilian, WANG Jing, LI Jianguang, GUO Dongliang. Genome-Wide Identification and Bioinformatics Analysis of DELLAs Gene Family in Longan[J]. Chinese Agricultural Science Bulletin, 2025, 41(5): 30-38.

Figures/Tables 7

References 31

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基因号&ID	开放阅读框	氨基酸数	外显子数	分子量	等电点		不稳定系数	跨膜结构域	信号肽		亚细胞定位
DlDELLA1 D.long000177.01	1802	599	4	67670.85	4.92		47.84	NO	NO		细胞核
DlDELLA2 D.long003121.01	1580	525	1	57490.43	5.41		43.58	NO	NO		细胞质
DlDELLA3 D.long006259.01	1811	602	1	68656.27	6.09		43.21	NO	NO		细胞质
DlDELLA4 D.long006260.01	1655	550	2	62939.07	5.7	43.53		NO	NO	细胞核
DlDELLA5 D.long033071.01	1151	382	1	42050.45	5.79	39.45		NO	NO	叶绿体
DlDELLA6 D.long035490.01	1727	574	3	62599.55	5.02	52.94		NO	NO	细胞核
DlDELLA7 D.long037337.01	1652	549	1	61772.8	5.08	47.07		NO	NO	细胞核
DlDELLA8 D.long027311.01	1745	580	1	64100.41	5.19	46.25		NO	NO	细胞质
DlDELLA9 D.long008983.01	1559	518	2	57581.08	6.26	48.25		NO	NO	细胞核
DlDELLA10 D.long029610.01	2007	668	1	73748.31	5.65	59.88		NO	NO	内质网

基因号&ID	开放阅读框	氨基酸数	外显子数	分子量	等电点		不稳定系数	跨膜结构域	信号肽		亚细胞定位
DlDELLA1 D.long000177.01	1802	599	4	67670.85	4.92		47.84	NO	NO		细胞核
DlDELLA2 D.long003121.01	1580	525	1	57490.43	5.41		43.58	NO	NO		细胞质
DlDELLA3 D.long006259.01	1811	602	1	68656.27	6.09		43.21	NO	NO		细胞质
DlDELLA4 D.long006260.01	1655	550	2	62939.07	5.7	43.53		NO	NO	细胞核
DlDELLA5 D.long033071.01	1151	382	1	42050.45	5.79	39.45		NO	NO	叶绿体
DlDELLA6 D.long035490.01	1727	574	3	62599.55	5.02	52.94		NO	NO	细胞核
DlDELLA7 D.long037337.01	1652	549	1	61772.8	5.08	47.07		NO	NO	细胞核
DlDELLA8 D.long027311.01	1745	580	1	64100.41	5.19	46.25		NO	NO	细胞质
DlDELLA9 D.long008983.01	1559	518	2	57581.08	6.26	48.25		NO	NO	细胞核
DlDELLA10 D.long029610.01	2007	668	1	73748.31	5.65	59.88		NO	NO	内质网