Wild Resources Investigation and Molecular Identification of Morels in Yongxing

doi:10.11924/j.issn.1000-6850.casb2025-0026

Abstract

Abstract:

In order to accurately master the wild morel species distributed in Yongxing Township, Chuxiong Prefecture and their occurrence patterns, to promote the long-term protection, utilization, cultivation and domestication research of local wild morel resources, a survey of wild morel resources was conducted at five locations including Naruan, Xiaozhuang, Guanyinsi, Huiba, and Lagu in the Yongxing area. Combined with the morphological characteristics of the fruiting bodies, molecular identification technology was used to conduct molecular identification and naming of the selected five samples. The results showed that the fruiting time of wild morels in the Yongxing area was from the end of September to the second half of October, and they mostly occurred 1-3 days after rainfall. The growth environment was mainly broad-leaved mixed forests dominated by oak trees and birch trees. Constructing a phylogenetic tree based only on ITS indicated that the five morel samples all belonged to the yellow morel group, but all species within the genus Morchella cannot be accurately distinguished. Using the multi-gene lineage consistency species identification method to construct a phylogenetic tree based on the combination of ITS+LSU+EF1-a+RPB1+RPB2 showed that the morels distributed in the Yongxing area were two yellow lineage species, Morchella sp. Mes-15 and Morchella sp. Mes-19, which was the first report.

Key words: Yongxing, wild morels, molecular identification, multiple gene, cultivation characteristics

LI Yinshuang, LI Xiaohua, CHEN Zhen, SU Yinsen, WANG Chunguang, LI Yanqiong, DOU Huaqiang, LI Qian, WANG Zuoqiang. Wild Resources Investigation and Molecular Identification of Morels in Yongxing[J]. Chinese Agricultural Science Bulletin, 2025, 41(18): 158-164.

Figures/Tables 5

References 26

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样本编号	分群	采集地点	采集时间	GenBank序列登录号
样本编号	分群	采集地点	采集时间	ITS	LSU	EF1-a	RPB1	RPB2
YD-01	黄色类群	那软	2023.9.27	PQ566680	PQ578194	PQ566701	PQ566691	PQ566696
YD-02	黄色类群	小庄	2023.9.27	PQ566681	PQ578195	PQ566752	PQ566692	PQ566697
YD-03	黄色类群	观音寺	2023.10.27	PQ566682	PQ578196	PQ566703	PQ566693	PQ566698
YD-04	黄色类群	灰坝	2023.10.16	PQ566683	PQ578197	PQ566704	PQ566694	PQ567699
YD-05	黄色类群	拉姑	2023.9.30	PQ566684	PQ578198	PQ566705	PQ566695	PQ566700

样本编号	分群	采集地点	采集时间	GenBank序列登录号
样本编号	分群	采集地点	采集时间	ITS	LSU	EF1-a	RPB1	RPB2
YD-01	黄色类群	那软	2023.9.27	PQ566680	PQ578194	PQ566701	PQ566691	PQ566696
YD-02	黄色类群	小庄	2023.9.27	PQ566681	PQ578195	PQ566752	PQ566692	PQ566697
YD-03	黄色类群	观音寺	2023.10.27	PQ566682	PQ578196	PQ566703	PQ566693	PQ566698
YD-04	黄色类群	灰坝	2023.10.16	PQ566683	PQ578197	PQ566704	PQ566694	PQ567699
YD-05	黄色类群	拉姑	2023.9.30	PQ566684	PQ578198	PQ566705	PQ566695	PQ566700