Isolation of Three Sphingomonas Strains from Sugar Beet Rotation Soil and Screening of Their Optimal Growth-Promoting Concentrations

doi:10.11924/j.issn.1000-6850.casb2026-0222

Abstract

Abstract:

The continuous cropping obstacle of sugar beet has become a prominent problem restricting the high-quality development of sugar beet industry in Heilongjiang Province. Excavating the beneficial microbial resources of rotation system is the key way to realize green prevention and control. This study aimed to screen Sphingomonas strains with plant growth-promoting potential from sugar beet rotation soils in Heilongjiang Province, in order to alleviate continuous cropping obstacles. Bacterial strains were identified based on Gram staining, 16S rDNA sequencing, and PCR amplification. Their capacities for phosphate solubilization, nitrogen fixation, potassium solubilization, and IAA production were evaluated. Pot experiments were conducted to determine the optimal growth-promoting inoculation concentrations. Three Sphingomonas strains were successfully obtained, namely W2 (Sphingobium abikonense), W9 (Sphingomonas panni), and W13 (Sphingomonas sp.). All three strains were capable of synthesizing IAA, with strain W13 exhibiting the highest yield (58.21 mg/L). Only strain W13 displayed preliminary evidence of nitrogen fixation potential, whereas none of the three strains demonstrated phosphate- or potassium-solubilizing activity. Pot experiments revealed that the optimal growth-promoting concentrations were 2.8×10⁹ CFU/mL for W2, 4.2×10⁹ CFU/mL for W9, and 1.0×10⁸ CFU/mL for W13. In summary, the three isolated sphingomonas strains possess plant growth-promoting characteristics and can be used as candidate strains for special microbial agents for sugar beet. In the future, compound application of strains, field control effect verification and growth-promoting molecular mechanism research can be carried out to provide new technical support for green prevention and control of sugar beet continuous cropping obstacles.

Key words: sugar beet, crop rotation, continuous cropping, Sphingomonas, plant growth-promoting bacteria, IAA, growth-promoting characteristics, pot experiment, microbial agent

CLC Number:

S154.39

CHENG Jianan, WANG Zenghao, GAO Youkai, FU Yihao, JIN Chengyu, SUN Yanchun. Isolation of Three Sphingomonas Strains from Sugar Beet Rotation Soil and Screening of Their Optimal Growth-Promoting Concentrations[J]. Chinese Agricultural Science Bulletin, 2026, 42(12): 38-47.

Figures/Tables 11

References 37

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Grope	CK	W2-0.2	W2-0.4	W2-0.6	W2-0.8	W2-1.0
Height(cm)	9.81±0.66c	11.35±0.66b	11.17±0.45b	13.83±0.87a	13.11±0.73a	13.20±0.69a
SD (cm)	2.93±0.28b	3.27±0.34a	3.24±0.24a	3.54±0.36a	3.53±0.38a	3.32±0.40a
DW (g)	0.11±0.01c	0.23±0.03b	0.19±0.03b	0.36±0.03a	0.33±0.04a	0.35±0.06a
RDW (g)	0.03±0.01d	0.07±0.01c	0.08±0.01c	0.14±0.03a	0.11±0.03b	0.09±0.02bc

Grope	CK	W2-0.2	W2-0.4	W2-0.6	W2-0.8	W2-1.0
Height(cm)	9.81±0.66c	11.35±0.66b	11.17±0.45b	13.83±0.87a	13.11±0.73a	13.20±0.69a
SD (cm)	2.93±0.28b	3.27±0.34a	3.24±0.24a	3.54±0.36a	3.53±0.38a	3.32±0.40a
DW (g)	0.11±0.01c	0.23±0.03b	0.19±0.03b	0.36±0.03a	0.33±0.04a	0.35±0.06a
RDW (g)	0.03±0.01d	0.07±0.01c	0.08±0.01c	0.14±0.03a	0.11±0.03b	0.09±0.02bc

Grope	CK	W9-0.2	W9-0.4	W9-0.6	W9-0.8	W9-1.0
Height(cm)	9.81±0.66c	11.39±0.65b	12.46±0.74a	12.80±0.87a	12.77±0.45a	12.53±0.58a
SD (cm)	2.93±0.28c	3.19±0.35bc	3.21±0.26bc	3.36±0.40ab	3.49±0.43ab	3.64±0.26a
DW (g)	0.11±0.01c	0.17±0.03bc	0.27±0.03b	0.39±0.11a	0.32±0.05ab	0.32±0.07ab
RDW (g)	0.03±0.01d	0.06±0.01c	0.09±0.01b	0.07±0.01bc	0.12±0.03a	0.07±0.01bc

Grope	CK	W9-0.2	W9-0.4	W9-0.6	W9-0.8	W9-1.0
Height(cm)	9.81±0.66c	11.39±0.65b	12.46±0.74a	12.80±0.87a	12.77±0.45a	12.53±0.58a
SD (cm)	2.93±0.28c	3.19±0.35bc	3.21±0.26bc	3.36±0.40ab	3.49±0.43ab	3.64±0.26a
DW (g)	0.11±0.01c	0.17±0.03bc	0.27±0.03b	0.39±0.11a	0.32±0.05ab	0.32±0.07ab
RDW (g)	0.03±0.01d	0.06±0.01c	0.09±0.01b	0.07±0.01bc	0.12±0.03a	0.07±0.01bc

Grope	CK	W13-0.2	W13-0.4	W13-0.6	W13-0.8	W13-1.0
Height(cm)	9.81±0.66d	14.39±0.36a	13.28±0.67b	12.88±0.66b	12.00±0.62c	13.11±0.85b
SD (cm)	2.93±0.28c	4.11±0.65a	3.54±0.38b	3.46±0.41b	3.43±0.43b	3.81±0.48ab
DW (g)	0.11±0.01d	0.48±0.03a	0.32±0.03b	0.25±0.04c	0.33±0.04b	0.35±0.08b
RDW (g)	0.03±0.01d	0.15±0.02a	0.11±0.01bc	0.08±0.01c	0.13±0.03ab	0.15±0.04a