In Silico Cloning and Characterization of Mastitis Resistant Gene Znf313 from Dairy Cows

Abstract

Abstract: In silico cloning was a new strategy of gene cloning developed with the development of genome, EST projects and bio-informatics. Using one differentially expressed EST in peripheral blood leukocytes of Holstein cows with mastitis as a querying probe, a novel cDNA sequence was obtained from bovine EST database of GenBank, which was named bovine zinc finger protein 313(Znf313) gene. The sequence of in silico cloning was verified by RT-PCR, and inserted into pGEM-T vector for sequencing. The bovine Znf313 gene cDNA contains a 693bp open reading frame, encoding 230aa protein, putative protein molecular weight is 25.67kD, isoelectric point is 6.90. The sequence has been submitted to GenBank with the accession number DQ010409.

CLC Number:

S823

Cao Suizhong, , , Li Hongbin, Yao Xueping, Zhao Xingxu, Du Lixin. In Silico Cloning and Characterization of Mastitis Resistant Gene Znf313 from Dairy Cows[J]. Chinese Agricultural Science Bulletin, 2007, 23(1): 6-006.

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In Silico Cloning and Characterization of Mastitis Resistant Gene Znf313 from Dairy Cows

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