Abstract: The Crofton weed (Eupatorium adenophorum Spreng.) is an alien species with strong invisibility. The successful invasion of this weed might be due to its strong ability of adaptation to the environment. This study was carried out in laboratory and field by using the method of experimental ecology, the effects of storage conditions, superstratum of the ground, pH and temperatures on the seed germination were observed systematically. It was found that the suitable environment conditions are required for seed germination of E. adenophorum. The seed germination could be deeply affected by superstratum of the ground, 5 mm superstratum could reduce the germination rate significantly and few seed could germinate when the ground was covered with 20mm of defoliation and deadwood. Seed germination rate could still amount to over 60% when seed is stored for one year under room temperature, but low temperature (5℃) and sand storage (5℃) can effectively lengthen longevity of the seed. The seed could adapt a wide range of pH and temperatures for germination, and the seed could germinate in the environment with pH ranging from 3.5 to 9.5 and temperatures ranging from 10℃ to 50℃. The results of this study had important reference values for explaining the invasion mechanism and discussing the defense mechanisms of ecosystems against the invasion of E. adenophorum.