Abstract: This experiment goal for optimizating the best soilless substrate of pot-strawberry, adapt pot-strawberry factorization production technical system, provides the practice and the theory basis for the correlation gardening plant soilless cultivation research and the factorization production. The trial studied the effect of growing and bearing in Toyonoka (Fragaria×Ananassa Duch)by different treatment with 11 combinations of sand, perlite, coal dreg and peat. One-Samples T Test results has evaluated the different substrate combination though the weighted average. We optimizated three better combinations, peat :coal dreg(volume ratio 1:1),perlite : coal dreg (1:1)and perlite :sand: coal dreg(1:1:1)