Abstract: 【Objective】Four different maize cultivars, nongda108, gaoyou115, jifeng58, zhengdan958, were chosen to study the effects of different harvested time on the yield of nutrient substance of corn stalk.【Method】The corn stalk were harvested at the growth days of the 92nd ,96th ,100th and 104th, respectively.【Results】The results indicated that there were obvious effects on the yield of organ matter, crude protein, fiber substance and digestible organ matter by different harvested time and different maize cultivars.【Conclusion】The results of trials indicate that the corn stalk could be harvested at the growth days of the 100th.