Abstract: Of 12 tomato lines, the analysis on the 4 fruit nourishment quality traits (the contents of vitamin C, total soluble solids and dry matter and ratio of sugar-acid ) was carried out to study the four kinds of disparities, which included the interspecific one, the one between two varieties in the same species , the one between the lines bred by different means in the same variety ,and the one between the different fruit-shaped lines in the same variety .The analytic method of orthogonal comparison of single degree of freedom was applied. The results showed that the line of Lycopersicum pimpinellifolium was better than the average of the lines of L.esculentum in the 4 traits at significant level of 1%, the average of the lines of var.cerasiforme was better than the average of the lines of var.vulgare in the 4 traits at significant level of 5% or 1%,and the two space mutation line(YH02-2 or YH02-6) was better than the average of the other lines of the same variation in the 4 trait, respectively, at significant level of 1%. Of the lines of var.cerasiforme , the lines with pear fruit were lower than the average of the lines with round-shaped or plum-shaped fruits in the 2traits(the contents of vitamin C and total soluble solids) , at significant level of 1%，the lines with plum-shaped fruits were higher than the average of the lines with round-shaped fruits, at significant level of 1%，and the lines with yellow-colored fruits(YH02-11) was lower than the average of the lines with red-colored fruits(YH02-1and YH02-7) in the trait of the contents of vitamin C. In conclusion, the best lines were YH02-12、YH02-2、YH02-6 and YH02-9, the average of the lines of var.cerasiforme was better than the average of the lines of var.vulgare in the 4 traits ,and the lines with pear fruit were lower than the average of the other lines of var.cerasiforme in the 4 traits.