Research of in vitro Cutting for Fast-Propagation on Stem of Hot Pepper

Abstract

Abstract: Although there were some advantage of short cycle and no difference between genotype on stem of hot pepper for fast-propagation, but the propagation coefficient was lower(1 times per cycle). For expanding propagation coefficient and overcoming influence between genotype in the course of cultivation, Stem of Niujiao pepper asepsis seeding was cultivated in this paper, and the fitting stem bud and rootage substrate were screened out. The effecte of asepsis stem for cultivation on difference source of color pepper was same to Niujiao pepper. The results showed that fitting substrate for in vitro cutting for fast-propagation on stem of hot pepper was MS mineral salt + improved MS organic matter + NAA 0.1mg/L + IAA0.2mg/L + CH 400g/L +AgNO3 3.5mg/L+ sucrose 30g/L + agar 7g/L, MS mineral salt +improved MS organic matter+ IAA 0.5mg/L + GA3 0.25mg/L+CH 400g/L + AgNO3 3.5mg/L+ sucrose 3g/L + agar 7g/L；rootage substrate was MS mineral salt + B5 organic matter + NAA 0.1 mg/L + IAA 0.2mg/L + AC 0.2%+ sucrose 30g/L + agar 7g/L.

CLC Number:

S604+.3

Zhu shinong, Cui qunxiang, Liu weidong. Research of in vitro Cutting for Fast-Propagation on Stem of Hot Pepper[J]. Chinese Agricultural Science Bulletin, 2007, 23(12): 274-274.

References

[1] 浙江农业大学主编.蔬菜栽培学各论(南方本)(第二版).北京:农业出版社,1985.
[2] 邹学校,马艳青,周群初,等.湘研辣椒种子的准时生产制[J].湖南农业大学学报,2001,27(3):192-196.
[3] 董兆龙,陈沁,刘文轩,等.辣椒子叶和下胚轴离体培养再生[J].上海大学学报(自然科学版),2003,(4):148-153.
[4] 李洪艳,王关林,姜丹,等.微型扦插快速繁殖辣椒试管苗的研究.辽宁师范大学学报(自然科学版).2002,25(3):296-297.
[5] Gunay A L, Rao R S. Invitro plant regeneration from hypocotyls and cotyledon explants of red pepper (Capsicum anniuum L.)[J].Plant Sci.Lett,1978(11):365-372.
[6] 缪武,刘志敏.辣椒离体培养研究进展[J].辣椒杂志(季刊).2005,(2):1-4.
[7] 邓明华,邹学校,周群初,等.辣椒子叶离体培养植株再生研究[J].长江蔬菜,2003,(6):36-39.
[8] 唐亮,刘文轩.辣椒再生体系的建立及基因工程研究进展[J].中国蔬菜.2003,(6):59-62.
[9] 刘卫东,崔群香,夏维东,等.彩色辣椒子叶离体培养技术的研究[J].扬州大学学报(农业与生命科学版),2003,24(1):63-66.
[10] 崔群香,朱士农,刘卫东.彩色辣椒子叶离体再生培养基的筛选[J].江苏农业科学,2005(2):73-74.
[11] 郝峥嵘,戚家华,王子霞,等.辣椒子叶的离体再生培养.[J]新疆农业科学,1993,(2):121-122.
[12] 葛扣麟,匡隈哲,田中正武.辣椒属不同野生种的组织培养与植株再生.上海农业学报,1991,7(4):10-16.
[13] Arroyo A L, Rao P S. In vitro plant regeneration from cotyledon and hypocotyls segments in two bell pepper cultivars.Plant cell Rep.1991,10(8):414-416.
[14] 崔群香,朱士农,刘卫东.彩色辣椒离体培养外植体及诱导培养基的筛选[J].金陵科技学院学报,2005,21(1):78-81.