Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (11): 27-31.

Special Issue: 生物技术 畜牧兽医

• 畜牧兽医科学 • Previous Articles     Next Articles

Molecular cloning and sequence analysis of Swine FcRn and its alternatively spliced variant

Li Haixia, Chen Liying, Liu Tao, Fan Pei, Qian Xin’an, Zhang Zhiqiang, Wang Yueying, Wang Yanling   

  • Received:2008-07-28 Revised:2008-08-13 Online:2008-11-05 Published:2008-11-05

Abstract: 【OBJECTIVE】To clone and analysis the FcRn gene of pig.【METHOD】Total RNA was extracted from mucosal tissue of duodenum and mRNA sequence of gene were amplified by RT-PCR using two designed primers. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of JM109. The sequence was analyzed to identify the recombinant plasmid.【RESULTS】Identity analysis showed that the two FcRn nucleotide sequence shared 99 % 【CONCLUSION】The porcine alternatively spliced variant of FcRn gene was successfully cloned in the present study and the sequence of spliced variant has been submitted to GenBank(Accession. EU852582)

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