Abstract: The osmotic pressures in subculture media and incubation temperatures were applied to explants during conservation in vitro in Fuji and Jonagold apple in order to achieve the optimal status for retardant growth. The results showed that a low incubation temperature can significantly retard the growth of explants. The optimal incubation temperature was 8℃ after an incubation at 25℃ for 5~10 d succeeding on inoculation of new plantlets, and these plantlets could be persevered for one and a half years with a survival rate of 100%. The increase of osmotic pressure in subculture media by enhancing the concentration of Agar, Sugar, and Mannitol could retard the growth of the explants. An increase of pressure retarded the amount of explant growth. But some explants died in media with a high concentration of Agar and Mannitol. The combination of osmotic pressure and temperature suggests that explants under incubation at a temperature at 8℃ and supplying 60 g/L Sugar in a medium would maintain their healthy status at the lowest growth rate. The effect had variation between cultivars with favorable results in Fuji when compared to Jonagold.