Studies on Genetic Transformation Conditions of Peanut Mediated by Agrobacterium tumefaciens

Abstract

Abstract: Applying to young leaflets regeneration system of peanut, genetic transformation of peanut using Agrobacterium-mediated approach were carrying on the research. The factors affecting the transformation, such as the selective pressure of antibiotic, pre-cultured, co-cultivation were examined. The optimized procedure was as following：after pre-cultured for 1-2 days，the young leaflets were dipped into an Agrobacterium suspension for 10-15 min，then co-cultivation for three days, the explants were transferred to selective media with 100mg/L Km and500mg/L Cef. These conditions benefited to improve the transformation efficiency. PCR analysis showed that transgenic peanut plants were obtained.

CLC Number:

S565.2

Sun Xianming, Wang Qiang, Yu Lizhi, Jiao yanlin, Gong qunying. Studies on Genetic Transformation Conditions of Peanut Mediated by Agrobacterium tumefaciens[J]. Chinese Agricultural Science Bulletin, 2008, 24(7): 218-220.

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Studies on Genetic Transformation Conditions of Peanut Mediated by Agrobacterium tumefaciens

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