Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (8): 69-73.

Special Issue: 园艺

• 农业生物技术科学 • Previous Articles     Next Articles

SRAP Molecular Marker and its Applications to Vegetable Crops

Zhao Guangwei, Xu Zhihong, Xu Yongyang   

  • Received:2008-04-23 Revised:2008-05-30 Online:2008-08-08 Published:2008-08-08

Abstract: SRAP(Sequence-Related Amplified Polymorphism)is a new molecular marker based on PCR technique,which combines simplicity,reliability,moderate throughput ratio and facile sequencing of selected bands. ORFs was amplified through the special primer set. The forward primers of 17 bp and reverse primers of 18bp amplified extrons and introns respectively.Polymorphism was produced since the varible introns,promoters and spacers among different individuals.The paper summarized SRAP molecular marker and its applications to genetic-map constructions,genetic diversity analysis, comparative genomic research , molecular marker and gene location of vegetable crops.

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