Abstract:

Abstract:【Objective】To separate dendritic cells( DC) without a requirement for in vitro propagation, which will lay a foundation for the study on the relations between the virus and swine DC. 【Methods】Skin layers were cut by hand with a blade and cultured overnight, and the isolated cells were acquired. The phenotype and morphology of DCs were identified by flow cytometry, Giemsa staining and scanning electron microscope. 【Results】The amount of skin-derived dendritic cells by way of cell counting was 5×106. These cells exhibited 82.3% rate of positive cells of CD1a and SWC3a antigens by flow cytometry (FCM), which was with one accord for specific molecular of DC; 90.2% rate of positive cells of CD80/86 was detected by FCM; There were more dendritic protrusions on the cells surface by way of Giemsa staining and scanning electron microscope. 【Conclusion】The skin-derived DCs of swine were isolated successfully according to the analysis of phenotype and morphology. The segregated swine skin-derived dendritic cells lay a foundation for the research on organ transplantation or immunosuppression mechanism of viral diseases in porcine.