Abstract:

Abstract: Mycelium growth characters of Gomphidius rutilus strains gained from wild fruitbodies by tissue separate were observed. Culture medium formula of the first-degree strains、the second-degree strains、the third-degree strains and liquid culture strains were screened. The results showed that the mycelium of the Gomphidius rutilus strains fully colonized in the PDA slant in 4-5 days under the temperature 25℃，fully colonized in the diameter 90mm PDA plate in 3 days，and still restored to growth under the temperature -18℃ in 3 days. The best medium formula of the first-degree strains was corn flour add PDA medium. The cottonseed hull or the pine forest soil add fermented corncob were more useful for the second-degree and the third-degree strains growth. All kind of PD medium were suitable for the growth of Gomphidius rutilus liquid culture strains. The mycelium were more densify and stronger on the medium add the corn flour、yeast and manure to crop extraction. Keywords: Gomphidius rutilus ; mycelium; medium