Long-term unreasonable application of phosphate fertilization is the limiting factor to the quality of flue-cured tobacco. The paper aims to understand phosphate fertilizer effect on soil and its influence on fluecured tobacco growth, and determine the best P formula. Effects of different types of phosphate fertilizer on soil chemical properties and flue-cured tobacco growth were studied by field experiment and soil testing with the DPS and SPSS software, and the single factor randomized block design. Researches on different phosphate fertilizer formula designs, soil chemical properties and flue- cured tobacco growth were conducted in three steps. First was the field design of different formulas of phosphate fertilizer, then was the soil sampling determination, determination of canopy structure index, chlorophyll, physical properties after baking smoke and root system, and finally were the analysis of variance and multiple comparison, and the establishment of the scheme of phosphate fertilizer formula. Variance analysis demonstrated that the contents of soil alkali hydrolysable nitrogen (F=7.9375, P=0.0068) and soil available potassium (F=6.2115, P=0.0145) and soil available phosphorus (F=5.9213, P=0.0158) and tobacco leaf area index (F=4.112, P=0.0433) and photosynthetic active radiation (F=4.6775, P=0.0378) in maturing stages of flue-cured tobacco were increased significantly by mono-ammonium phosphate, physical characters of flue-cured tobacco were improved, it was one of the effective measures to prevent and control soil acidification, and was favorable to root growth of fluecured tobacco. The contents of soil alkali hydrolyzable nitrogen (F=5.112, P=0.0237) and soil available phosphorus (F=5.20, P=0.0177) and tobacco leaf area index (F=3.9621, P=0.0495) were increased significantly by high phosphorus basal fertilizer type II, and soil pH (F=4.2351, P=0.0411) was decreased significantly, it was favorable to root growth of flue- cured tobacco. The contents of soil organic matter were increased by high phosphorus basal fertilizer type I, high phosphorus basal fertilizer could promote the growth and maturity of flue-cured tobacco, improve leaf area index of flue-cured tobacco in vigorous growth stages and maturing stages by 1.26%-31.69%, and improve photosynthetic active radiation of flue-cured tobacco in maturing stages by 2.04%- 11.51% . This is of great significance in directing fertilizer application in the phosphorus deficiency soil in Xiangxi Zhou.