水稻品种‘连粳7号’对黑条矮缩病的抗性分析

doi:10.11924/j.issn.1000-6850.2013-2161

中国农学通报 ›› 2014, Vol. 30 ›› Issue (12): 51-56.doi: 10.11924/j.issn.1000-6850.2013-2161

所属专题：水稻；农业生态

水稻品种‘连粳7号’对黑条矮缩病的抗性分析

王宝祥谭维娜卢百关宋兆强徐大勇

收稿日期:2013-08-12 修回日期:2013-09-03 出版日期:2014-04-25 发布日期:2014-04-25
基金资助:
江苏省自然科学基金“水稻黑条矮缩病抗性资源筛选和抗性基因(QTL)定位”(SBK201122073)；江苏省农业科技自主创新资金“抗黑条矮缩病水稻品种资源筛选、抗性基因定位及多基因聚合育种研究，抗黑条矮缩病水稻新种质创制”[CX(12)5112，1003-2]；连云港市科技攻关计划超高产优质多抗广适中粳水稻新品种选育(农业，CN1201)；国家水稻产业体系“水稻病虫草害预警及关键防控技术集成与示范”(CARS-01-02A)。

Resistance Analysis of the Rice Cultivar ‘Lianjing 7’ to Rice Black-streaked Dwarf Disease

Received:2013-08-12 Revised:2013-09-03 Online:2014-04-25 Published:2014-04-25

摘要/Abstract

摘要： 为分析水稻品种‘连粳7号’对水稻黑条矮缩病的抗性，获得与抗性基因紧密连锁的分子标记，通过田间自然接种和室内人工接种方法，对‘连粳7号’及其与感病品种‘培矮64’衍生来的F_2:3群体进行黑条矮缩病抗性鉴定。结果表明，‘连粳7号’发病率最高为12.25%，最低为5.5%。‘连粳7号’的亲本中，仅‘中粳川-2’的抗性与‘连粳7号’相近。另外，‘连粳7号’对灰飞虱不具有抗性，所以‘连粳7号’对黑条矮缩病的抗性是品种本身对病毒的抗性所致。连粳7号/培矮64 F_2:3群体中，各个家系表型呈现连续分布，并具有超亲分离特点，说明抗性由多基因控制。最终，通过BSA法获得与抗性位点紧密连锁的标记RM287。综上，‘连粳7号’对黑条矮缩病具有较强抗性，抗性来自亲本‘中粳川-2’，标记RM287将为利用分子标记辅助培育抗黑条矮缩病水稻新品种提供有力工具。

关键词: 食用, 食用

Abstract: To analyze the resistance of‘Lianjing 7’against rice black-streaked dwarf disease, and find the linked marker with the resistant gene, the resistance analysis of‘Lianjing 7’, its parents and the F_2:3 population, which derived from a cross between‘Pei’ai 64’, a susceptible variety, and‘Lianjing 7’, a variety with resistance to RBSDS, were evaluated using natural infection methods and artificial inoculation by scoring the disease rating. The result showed‘Lianjing 7’was resistant to RBSDD with the disease rate between 5.5% and 12.25% in all test. In the parents of‘Lianjing 7’, only‘Zhongjingchuan-2’was resistant to RBSDD. The antixenosis and antibiosis score of‘Lianjing 7’was similar to the susceptible variety‘Wuyujing 3’, so the resistance of‘Lianjing 7’was mainly caused by the resistance to the RBSDV. In the Lianjing 7/Pei’ai 64 F_2:3 population, the disease rates showed a continuous distribution with transgressive segregation, it indicated the resistance of‘Lianjing 7’was controlled by polygenic. Finally, we got the marker RM287 closely linking with the resistance loci by BSA. To sum up,‘Lianjing 7’was resistant to RBSDD, and it acquired the resistance of‘Zhongjingchuan-2’. The marker RM287 should be useful for breeding new RBSDD-resistant cultivars by marker-assisted selection (MAS).

王宝祥谭维娜卢百关宋兆强徐大勇. 水稻品种‘连粳7号’对黑条矮缩病的抗性分析[J]. 中国农学通报, 2014, 30(12): 51-56.

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水稻品种‘连粳7号’对黑条矮缩病的抗性分析

Resistance Analysis of the Rice Cultivar ‘Lianjing 7’ to Rice Black-streaked Dwarf Disease

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