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中国农学通报 ›› 2008, Vol. 24 ›› Issue (8): 74-79.

所属专题: 小麦

• 生物技术科学 • 上一篇    下一篇

六倍体小黑麦HMW-GS亚基分析

高居荣,封德顺,李兴锋,王洪刚   

  • 收稿日期:2008-04-30 修回日期:2008-06-13 出版日期:2008-08-08 发布日期:2008-08-08

Analysis of High Molecular Weight Glutenin Subunits in Hexaploid Triticale

Gao Jurong, Feng Deshun, Li Xingfeng, Wang Honggang   

  • Received:2008-04-30 Revised:2008-06-13 Online:2008-08-08 Published:2008-08-08

摘要: 为有效利用引自国际玉米小麦改良中心的175份六倍体小黑麦材料,采用SDS-PAGE电泳方法对其高分子量麦谷蛋白亚基(HMW-GS)组成进行了分析。结果表明,这些材料的HMW-GS类型丰富,在Glu-A1,Glu-B1和Glu-Rx三个位点上分别检测到3种、4种和7种不同的亚基类型;在Glu-A1位点Null 亚基出现的频率最高,为49.14%,其次为1和2* 频率分别为30.29%和20.57%;在Glu-B1位点13+16亚基出现的频率最高,为45.71 %;黑麦染色体编码的亚基主要是21*和22*。共检测到27种HMW-GS组合类型,其中(N,21*,13+16)、(N,21*,14+15,22*)、(1,21*,13+16,22*)、(N,21*,7+8)和(1,21*,13+16)出现的频率较高,分别为10.29%、9.14%、8.57%、6.29%和5.71%。

关键词: 链霉菌, 链霉菌, 作用机制, 生物防治

Abstract: The composition of high molecular weight glutenin subunits (HMW-GS) in 175 hexaploid Triticale from CIMMYT were analyzed using SDS-PAGE method in this experiment. The result showed that the HMW-GS composition of 175 lines were extremely abundant, 3 ,4 and 7 different HMW-GS types were found in the loci of Glu-A1,Glu-B1 and Glu-Rx respectively . The frequency of subunit Null was the highest(49.14%) at Glu-A1 locus, and the second are 1 (30.29%) and 2* (20.57%) .(13+16)was the highest (45.71%) at Glu-B1. The subunits coded by rye chromosome were mainly 21* and 22*. 27 HMW-GS composition types were found ,and among them the frequency of(N,21*,13+16,)(10.29%)、(N,21*,14+15,.22*)(9.14%)、(1,21*,13+16,22*)(8.57%)、(N,21*,7+8)(6.29%)和(1,21*,13+16)(5.71%)was higher.

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