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中国农学通报 ›› 2010, Vol. 26 ›› Issue (18): 170-174.

所属专题: 生物技术 园艺

• 农学 农业基础科学 • 上一篇    下一篇

根结线虫侵染对黄瓜生长和内源激素含量的影响

刘庆安 夏凯 叶梅荣   

  • 收稿日期:2010-02-07 修回日期:2010-06-08 出版日期:2011-09-20 发布日期:2011-09-20
  • 基金资助:
    安徽科技学院引进人才科研经费专项

Effects of Meloidogyne spp. on the growth and endogenous hormone contents of cucumber(Cucumis sativus L.)

  • Received:2010-02-07 Revised:2010-06-08 Online:2011-09-20 Published:2011-09-20

摘要: 笔者通过测定根结线虫侵染后48小时内的黄瓜体内的内源植物激素含量的变化,研究侵染初期各内源植物激素之间的动态平衡状态,以探索黄瓜根结线虫病害产生的逆境应答及生理机制,为研究植物的诱导抗病性和根结线虫防治提供理论参考。试验结果表明,根结线虫侵染后,黄瓜生长缓慢,植株矮小,提前衰老。通过酶联免疫吸附法测定根结线虫侵染48小时内的黄瓜内源激素含量变化发现,黄瓜叶片中茉莉酸(JA)的含量在线虫侵染16小时内急剧上升,上升幅度为对照的8.2倍;水杨酸(SA)、脱落酸(ABA)和异戊烯基腺苷(iPAS)在侵染后含量有显著上升的趋势,并在24小时分别达到各自的峰值;但是生长素(IAA)和赤霉素(GA1+3)含量仅有短暂升高过程,其余时间均显著低于对照。

关键词: 丹参, 丹参, 脂溶性成分, 水溶性成分, 土壤因子, 土壤结构, 土壤理化性质

Abstract: By measuring the root-knot nematode infection of cucumber within 48 hours after the endogenous plant hormone content changes, we studies the initial stage of infection between the endogenous plant hormone homeostasis, analysis the stress response and physiological mechanisms of cucumber root-knot nematode disease, in order to find the induction of plant disease resistance theoretical reference. The results show that when cucumber plant was infected by Meloidogyne spp., the height, the fresh weight of the above-ground part and the root were decreased significantly. By enzyme-linked immunosorbent assay(ELISA),we analyzes the changes of endogenous hormones content four hours a time till 48 hours after the inoculation. Results indicated that the content of Jasmonic acid (JAs) in the leaf of infected plant was increased to 820% of that in the leaf of control plant during 16 hours after the inoculation。The contents of Salicylic acid (SA), Abscisic acid(ABA)and iPAs were also increased significantly after the inoculation, they all achieved their peak values 24 hours after the inoculation. While the content of IAA and gibberellin1+3(GA1+3) were lower than that in control plant except transitory increase during the 48 hours.