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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (1): 24-29.doi: 10.11924/j.issn.1000-6850.2011-2016

所属专题： 生物技术； 农业气象

• 林学 园艺 园林 • 上一篇    下一篇

白刺对干旱胁迫的生理生化反应

李毅 屈建军 张伟民 牛清河 葛君州 徐鹏 安黎哲   

  • 收稿日期:2011-07-11 修回日期:2011-08-19 出版日期:2012-01-05 发布日期:2012-01-05
  • 基金资助:

    极端干旱区荒漠植被种群繁殖对策的研究

Physiological and Biochemical Responses of Nitraria L. to Drought Stress

  • Received:2011-07-11 Revised:2011-08-19 Online:2012-01-05 Published:2012-01-05

PDF (PC)

978

摘要：

研究白刺幼苗抗旱特性的生理学机制，期望为干旱农业生产提供理论依据。实验采用盆栽，用控制浇水的方法对不同强度的干旱胁迫梯度上对白刺幼苗叶片相对含水量、丙二醛、脯氨酸、可溶性蛋白质、叶绿素含量、超氧离子自由基产生速率和抗氧化酶活性的变化特点进行了研究。试验结果表明：在干旱胁迫下，白刺叶片相对含水量降低、脯氨酸含量呈增加趋势；可溶性蛋白质、叶绿素含量在轻度和中度干旱胁迫时上升，在重度干旱胁迫时显著下降；随着干旱胁迫的加剧，膜透性上升，丙二醛含量和超氧离子自由基产生速率增加。抗氧化系统酶活性测定结果表明，SOD、POD、CAT 3个酶能配合协同作用，降低膜脂过氧化程度，减少水分胁迫造成的伤害。

关键词: 小麦, 小麦, 蚕豆, 间作, 硅, 小麦白粉病

Abstract:

The aim was to study the drought resistance mechanism of Nitraria L., and provide theoretical basis for agricultural production. In order to assess the ability of tolerating drought in Nitraria L., we tested the effects of drought stress on physiological and biochemical of Nitraria L. seedlings. In greenhouse experiment we study the changes of relative water content (RWC), malondialdehyde (MDA), proline, soluble protein, Chl content, rate of producing O2-, and the activity of SOD, POD and CAT. It was found: under drought stress, relative water content decreased white proline content showed an increasing trend; in mild and moderate drought stress the soluble protein and chlorophyll content increased, but in severe drought stress they all significantly decreased; as the drought stress, membrane permeability increased, MDA content and rate of producing O2- increases, too. Antioxidant system activity test showed that SOD, POD, CAT activity can synergies, reduce membrane lipid peroxidation and the damage caused by water stress.