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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (15): 31-35.doi: 10.11924/j.issn.1000-6850.2011-3282

所属专题： 棉花

• 农学 农业基础科学 • 上一篇    下一篇

不同植物生长调节剂处理对陆地棉子叶节再生的影响

贾佩佩 胡根海 张金宝 王清连   

  • 收稿日期:2011-11-07 修回日期:2011-12-29 出版日期:2012-05-25 发布日期:2012-05-25

Different Plant Growth Regulator Treatment on the Regeneration of Upland Cotton Cotyledon Sections

  • Received:2011-11-07 Revised:2011-12-29 Online:2012-05-25 Published:2012-05-25

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摘要：

为了建立高效的棉花子叶节再生体系用于遗传转化，以3个陆地棉品种的子叶节为材料，对棉花子叶节再生体系进行了优化，特别对子叶节诱导生根进行了探讨。结果表明：在子叶节丛生芽诱导中，不同材料的最佳诱导培养基存在差异，‘百棉1号’适宜的培养基为MSB+1.0 mg/L 6-BA +0.5 mg/L IBA；‘百棉2号’适宜的培养基为MSB +2.5 mg/L 6-BA +2.5 mg/L KT；‘珂字312’适宜的培养基为MSB +2.0 mg/L 6-BA，每个外植体可以得到3~4个芽，且大部分芽都可以伸长。生根培养基为MSB+0.5 mg/L NAA。

关键词: 稳定性, 稳定性

Abstract:

To establish a high frequency regeneration system for the gene transformation of cotton, cotyledon nodes of three cotton varieties were acted as the explants, regeneration of cotton cotyledon section was modified and optimized to, especially the cotyledon node rooting was discussed. The results showed that: For bud induction, the best inductive medium suitable for ‘Baimian 1’ was MSB + 1.0 mg/L 6-BA and 0.5 mg/L IBA; the MSB + 2.5 mg/L 6-BA + 2.5 mg/L KT was suitable for ‘Baimian 2’. The MSB medium supplemented with 2.0 mg/L 6-BA was appropriate for ‘Coker 312’. Each explant could obtain 3 to 4 buds, and most of the buds could be elongate. For rooting, MSB+0.5 mg/L NAA medium was utilized.