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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (15): 158-162.doi: 10.11924/j.issn.1000-6850.2011-3593

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

高产核酸酶菌株的分离、筛选与诱变育种

田利飞 涂璇 龚大春 姚鹃 陈君芝   

  • 收稿日期:2011-12-01 修回日期:2012-01-12 出版日期:2012-05-25 发布日期:2012-05-25
  • 基金资助:

    三峡大学博士启动基金

Screening and Mutation Breeding of Strains Preducing Nucleic Acid Enzymatic

  • Received:2011-12-01 Revised:2012-01-12 Online:2012-05-25 Published:2012-05-25

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摘要：

为获得具有自主知识产权，满足生产需求的高产核酸酶P1的菌株。以2%RNA作为唯一碳、氮源的筛选培养基，从桔园土壤中初步筛选出65株具有产生核酸酶P1的菌株。结果表明：以其中酶活较高的YC34号菌株为出发菌株，该菌株的初始酶活性为127.3 U/mL，通过紫外诱变、微波诱变、LiCl诱变3种方式依次进行复合诱变处理后，获得酶活最高的突变株YC34-1，其粗发酵液的酶活达到360.7 U/mL，较原始出发菌株酶活增长了183.3%，具有良好的应用开发潜力。经初步形态学鉴定该菌株可归属为青霉属柑桔青霉(Penicillium citrinum)。

关键词: 提取, 提取

Abstract:

For possessing independent intellectual property rights and meeting the needs of high-yield production strains of nuclease P1, 2%RNA was used as the unique carbon and nitrogen sources of the screening culture medium, 65 strains which could produce nuclease P1 were screened from citrus orchard soil. Strain YC34 was used as the mutation breeding starting strain with the highest enzyme activity to 127.3 U/mL, after treated by complex mutagenesis of UV, microwave and LiCl mutation, the high-yield strainYC34-1 was obtained. The crude nuclease P1 activity of broth was increased to 360.7 U/mL, 183.3% higher than the original strain YC34, which had good potential in application. The preliminary morphological results showed that the strain YC34 was identified to be Penicillium citrinum.