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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (21): 164-168.doi: 10.11924/j.issn.1000-6850.2011-3867

• 生物技术科学 • 上一篇    下一篇

拟南芥AtNUDT10启动子的分离及其功能分析

张秀春 李若霖 唐文 夏亦荠 彭明 吴坤鑫   

  • 收稿日期:2011-12-21 修回日期:2012-02-16 出版日期:2012-07-25 发布日期:2012-07-25
  • 基金资助:

    中国热带农业科学院热带生物技术研究所中央级公益性科研院所基本科研业务费专项

Cloning and Function Analysis of Promoter of AtNUDT10 in Arabidopsis thaliana

  • Received:2011-12-21 Revised:2012-02-16 Online:2012-07-25 Published:2012-07-25

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摘要：

根据已发表的拟南芥基因组序列设计合成一对引物，以拟南芥基因组DNA为模板克隆AtNUDT10上游非编码区，并与pBAR-GUS3相连构建了植物表达载体pNUDT10P-GUS，采用农杆菌GV3101介导的渗透法转化野生型拟南芥，通过除草剂筛选获得了一批抗性纯合子转基因植株。然后对转基因植株2周幼苗进行GUS活性的组织化学染色分析，并对3.5~4周的转基因植株叶片进行病原菌诱导表达分析。结果表明，已获得AtNUDT10启动子，该启动子为组成型表达的启动子，并且病原菌Pst.DC3000及Pst.DC3000 AvrB对该启动子没有诱导作用。

关键词: 土壤养分, 土壤养分

Abstract:

5’-UTR of AtNUDT10 was cloned by PCR from Arabidopsis thaliana and was inserted into pBAR-GUS to form a recombined construct pNUDT10P-GUS. The construct was then used to transform wide-type Arabidopsis thaliana by Agrobacterium tumefaciens mediating. A batch of transgenic Arabidopsis thaliana with 5’-UTR of AtNUDT10 was obtained through glufosinate screening. The results showed that, we had got the promoter of AtNUDT2 that was constitutive promoter. Furthermore, the expression of the promoter could not be induced by the Pst.DC3000 and Pst.DC3000 AvrB.

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