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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (19): 210-214.doi: 10.11924/j.issn.1000-6850.2012-1224

• 林学 园艺 园林 • 上一篇    下一篇

栀子优良品种快繁体系的建立

崔思然 陈彩霞 江艳华 李艾莲   

  • 收稿日期:2012-03-31 修回日期:2012-05-26 出版日期:2012-07-05 发布日期:2012-07-05

Studies on Tissue Culture and Rapid Propagation of Superior Characteristics of Gardenia jasminoides Ellis

  • Received:2012-03-31 Revised:2012-05-26 Online:2012-07-05 Published:2012-07-05

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摘要：

为了保存和尽快繁殖栀子优良种质，以品系优良的10年生栀子新萌枝条为试验材料，在添加不同植物激素的MS培养基上进行了栀子组培再生体系建立的试验研究，并探讨栀子组培苗的炼苗移栽技术。结果表明：适宜栀子外植体的启动培养基为MS+6-BA 1.5 mg/L+NAA 0.5 mg/L；增殖培养基为MS+6-BA 1.5 mg/L+NAA 0.3 mg/L，增值系数可达4.6；较适宜的生根培养基为1/2MS+IBA 2.0 mg/L，生根率为87%；炼苗移栽基质使用草炭和蛭石，比例为1∶1时效果最好。

关键词: 土壤养分, 土壤养分

Abstract:

To establish a new technique for in vitro rapid propagation and conservation of superior germplasm of Gardenia jasminoides Ellis, new emerging stems of a ten-year old Gardenia were used as explants to develop an efficient protocol for tissue-culture propagation based on MS medium supplemented with different hormones, in addition to research into the transplanting technique. Results indicated that the proper original induction medium was MS+6-BA 1.5 mg/L+NAA 0.5 mg/L; Shoot reproduction medium was MS+6-BA 1.5 mg/L+IBA 0.3 mg/L with a propagation ratio of 4.6; The best rooting medium was 1/2MS+IBA 2.0 mg/L, and the rooting rate was 87%; The best growth in the matrix was based on the ratio of peat∶vermiculite =1:1(V:V).