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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (25): 21-25.doi: 10.11924/j.issn.1000-6850.2013-0689

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

水曲柳愈伤组织诱导及悬浮细胞体系的建立

丁小萌 闫俊崎 周博 齐凤慧 詹亚光   

  • 收稿日期:2013-03-13 修回日期:2013-04-23 出版日期:2013-09-05 发布日期:2013-09-05
  • 基金资助:
    十二五支撑项目：白桦水曲柳新品种选育技术研究;东北林业大学生命科学院创新实验项目“水曲柳悬浮培养体系的研究”

Callus induction and establishment of cell suspension culture system for Fraxinusm andshu rica

  • Received:2013-03-13 Revised:2013-04-23 Online:2013-09-05 Published:2013-09-05

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摘要： 水曲柳悬浮细胞培养比较困难，本研究首次获得水曲柳悬浮细胞。以水曲柳叶柄为材料诱导愈伤组织，通过2,4-D浓度的筛选，建立稳定的水曲柳悬浮细胞培养体系，可为水曲柳悬浮细胞系遗传转化或诱变后单克隆的筛选提供材料。水曲柳愈伤组织在WPM+BA0.1mg/L+2,4,-D0.015mg/L+20g/L蔗糖液体培养基中振荡培养3-4代，可获得稳定的悬浮细胞系，最佳继带周期为15d。

关键词: 抑菌活性, 抑菌活性

Abstract: A stable suspending cell culture of Fraxinusm andshu rica could be used for genetic transformation or screening monoclone after mutagenesis. In this study, stalks of Fraxinusm andshu rica were used to induce calli and suspending cells were obtained after a series gradient of 2,4-D were added in the culture. The calli could be subcultured 3-4 gengerations every 15d in the media of WPM+BA0.1mg/L+2,4,-D0.015mg/L+20g/L sucrose to get stalbe cell culture.