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中国农学通报 ›› 2014, Vol. 30 ›› Issue (15): 298-304.doi: 10.11924/j.issn.1000-6850.2013-2441

所属专题: 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

传统豆酱发酵过程中霉菌的形态学分析

孙雯 葛菁萍   

  • 收稿日期:2013-09-12 修回日期:2013-12-23 出版日期:2014-05-25 发布日期:2014-05-25
  • 基金资助:
    国家自然科学基金“菌群演替与温水沤麻系统关键酶产生菌代谢组学特征的耦合机制”(31270534);国家自然科学基金青年科学基金“酸菜发酵生态系统中细菌群落与代谢物组特征耦合机制(31300355)”;黑龙江大学高层次人才(创新团队)支持计划“功能微生物选育及其产物应用”(Hdtd2010-17);黑龙江省高等学校科技创新团队(农业微生物发酵技术,2012td009)。

Morphological Analysis of Fungus from the Traditional Fermented Soybean Paste

  • Received:2013-09-12 Revised:2013-12-23 Online:2014-05-25 Published:2014-05-25

摘要: 本试验是以传统微生物学方法对自然发酵豆酱中的霉菌进行形态学分析,初步探究豆酱发酵过程中霉菌菌群的变化规律,探索在传统豆酱发酵过程中霉菌的组成及优势菌株,这对于豆酱发酵起到关键作用。对不同发酵时间的豆酱样品中的霉菌进行计数、分离,并通过载片培养法对其进行鉴定。利用传统分离培养的方法从传统发酵豆酱中分离得到6株具有代表性的霉菌,对每类中具有代表性的菌株进行进一步的真菌湿室培养法鉴定后,确定了其种属地位,分别为:米曲霉、大毛霉组、犁头霉属、总状枝毛霉组、雅致放射毛霉组和曲霉属,其中米曲霉是优势菌。本研究揭示了豆酱发酵过程中霉菌的菌相组成,这为将豆酱生产由传统制备转向工厂化生产提供了菌株基础。

关键词: 相关性, 相关性

Abstract: This experiment was based on traditional microbiological methods for natural mold morphology analysis of fermented bean paste, delving into the law of the molds change by natural fermentation, exploring the mold components and the dominating ones in the traditional soybean fermentation, which played a key role for the sauce fermentation. In this paper, the mold in the bean paste sample of different fermentation time were counted and separated, and identification by the slide culture method. Six representative strains were isolated from the traditional fermentation bean paste by using traditional cultivation and further to identify by fungi wet chamber culture method, determining the species status, respectively: Aspergillus oryzae, Mucedo, Absidia, Mucor racemosus, Actinomucor elegans, Aspergillus and Aspergillus oryzae became the dominating one. This study revealed the mold components in the process of the bean sauce fermentation, which would provide the basis from the traditional preparation to factory production.