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中国农学通报 ›› 2014, Vol. 30 ›› Issue (32): 26-31.doi: 10.11924/j.issn.1000-6850.2014-0399

所属专题: 生物技术 水产渔业

• 水产 渔业 • 上一篇    下一篇

鱼腥草单方制剂对草鱼幼鱼生长及生理生化指标的影响

谭娟,邓雨飞,曹宇舰,贺三喜,陈志超,易凡,马业新   

  1. 怀化学院生命科学系,怀化学院生命科学系,怀化学院生命科学系,怀化学院生命科学系,怀化学院生命科学系,怀化学院生命科学系,怀化学院生命科学系
  • 收稿日期:2014-02-20 修回日期:2014-02-20 接受日期:2014-05-19 出版日期:2014-11-20 发布日期:2014-11-20
  • 通讯作者: 谭娟
  • 基金资助:
    民族药用植物资源研究与利用湖南省重点实验室委托项目资助“复方中草药添加剂对黄颡鱼非特异性免疫和抗氧化功能的影响” (HHUW2011-62);湖南省重点学科建设项目资助“植物学”(2011-42)。

Effects of Houttuynia cordata Single Preparations in Feed on Growth and Physiological and Biochemical Index of Juvenile Grass carp (Ctenopharyngodon idellus)

  • Received:2014-02-20 Revised:2014-02-20 Accepted:2014-05-19 Online:2014-11-20 Published:2014-11-20

摘要: 为了提高草鱼幼鱼的抵抗力和存活率,研究不同剂量的鱼腥草单方制剂在不同时间对草鱼幼鱼生长及生理生化指标的影响,为鱼腥草作为饲料添加剂开发利用提供理论依据。本试验将鱼腥草细粉分别按质量分数1%、2%、3%的比例添加到基础饲料中,连续投喂草鱼幼鱼(10.83±0.57) g 90 天,每30 天取样,分别测量生长指标、肌肉成分以及溶菌酶和SOD等生理生化指标。结果表明:与对照组相比,2%试验组显著提高了草鱼的尾均重和增重率,并在30、60、90 天都显著高于1%和3%试验组(P<0.05)。各试验组均能提高草鱼溶菌酶活性和NO的含量,但只有2%试验组投喂60 天时能显著提高草鱼机体内溶菌酶活性和NO含量(P<0.05),但对不同脏器亦存在作用时间的差别。各试验组的SOD活性均显著高于对照组(P<0.05),且随着作用时间延长逐渐被诱导升高。各试验组GOT和GPT的活性均低于对照组,且GPT活性与对照组存在显著性差异(P<0.05),表明其对肝细胞有一定的保护作用。综合研究认为:鱼腥草单方中草药饲料添加剂的适宜投喂周期为30~60天,适宜添加剂量是2%。

关键词: 敏感性, 敏感性

Abstract: In order to improve the resistance and survival rate of juvenile grass carp (Ctenopharyngodon idellus ), the study investigated the effects of different doses ofHouttuynia cordata single preparations on the growth performance and physiological and biochemical indexes of juvenile grass carp after fed of different time, it could provide a theoretical guide forHouttuynia cordata as a feed additive to development and utilization. TheHouttuynia cordata powder added to the basic feed in proportion to the mass fraction of 1%, 2% and 3%. Grass carp individuals, the average weight of which was (10.83±0.57) g, fed of different concentrations of Houttuynia cordata single preparations. After fed of 30d, 60d and 90d, its growth index, muscle composition, lysozyme and SOD and other physiological and biochemical indexes were measured. Results showed: average weight and weight gain rate in group 2 increased significantly compared with those of the control group, and significantly higher than group 1 and group 3 at 30d, 60d and 90d(P <0.05). Every group could increase the lysozyme activity and NO content of grass carp, but only group 2 which fed 60d could significantly improve the lysozyme activity and NO content(P <0.05), but there was different reaction time between different organs. The SOD activity in every test group was much higher than those of the control group (P<0.05), and it gradually increased with the reaction time prolonged. The GOT and GPT activity in every test group were lower than those of the control group, and the GPT activity had significant difference between test groups and control group (P <0.05), so it showed some certain protective effect for liver cell. In a word, the optimum feeding time of Houttuynia cordata single Chinese herbal medicine feed additive was 30d to 60d and the optimum additive volume was 2%.