浙江地区柑桔炭疽病对甲基硫菌灵的抗性水平及其分子机制

doi:10.11924/j.issn.1000-6850.2014-2149

中国农学通报 ›› 2015, Vol. 31 ›› Issue (17): 90-94.doi: 10.11924/j.issn.1000-6850.2014-2149

所属专题：农业生态

浙江地区柑桔炭疽病对甲基硫菌灵的抗性水平及其分子机制

蒲占湑

浙江省柑桔研究所

收稿日期:2014-08-07 修回日期:2015-05-27 接受日期:2015-04-22 出版日期:2015-07-27 发布日期:2015-07-27
通讯作者: 蒲占湑
基金资助:
浙江省农业科学院提升工程项目 “柑桔炭疽病菌的遗传多样性及对苯并咪唑类药剂的抗性机理研究” (2013R27Y01E01)。

Resistance Level and Mechanism of Colletotrichum gloeosporioides to Thiophanate-Methyl in Zhejiang

Received:2014-08-07 Revised:2015-05-27 Accepted:2015-04-22 Online:2015-07-27 Published:2015-07-27

摘要/Abstract

摘要： 了解浙江地区柑橘炭疽病菌株对甲基硫菌灵的抗药性频率及其抗性分子机制。采用最低抑制浓度(MIC)法测定甲基硫菌灵对62株炭疽病菌株的敏感性，并利用PCR扩增的方法扩增12株代表性菌株的β-微管蛋白基因。结果表明：分离获得的菌株中，对甲基硫菌灵高抗菌株56株(90.32%)，敏感菌株6株(9.68%)。通过对12株代表性的炭疽病菌株部分β-微管蛋白基因序列的分析。结果显示：甲基硫菌灵的抗性菌株发生单一的核苷酸突变(A→C)，导致抗性菌株(HR)核苷酸所编码的198位氨基酸由敏感菌株的谷氨酸(GAG)突变为丙氨酸(GCG)。柑橘炭疽病菌对甲基硫菌灵的抗药性与β-微管蛋白198位氨基酸突变有关。

关键词: 毒性, 毒性

Abstract: The study is to observe the resistant frequency and the molecular mechanism of Colletotrichum gloeosporioides to thiophanate-methyl in Zhejiang. The sensitivity to thiophanate-methyl of 62 isolates of C. gloeosporioides was determined by the method of minimum inhibitory concentration (MIC). The β-tubulin gene region of 12 isolates was amplified. The results showed that 56 of 62 isolates (90.32%) were highly resistant to thiophanate- methyl, and 6 isolates (9.68% ) were sensitive. The β- tubulin sequences of 12 representative isolates of C. gloeosporioides were aligned. It showed that the resistant isolates to thiophanate-methyl produced mutation (A→C), and glutamic acid (GAC) was substituted by alanine (GCG) at amino acid 198. The point mutation of amino acid at codon 198 of C. gloeosporioides β-tubulin gene was related to thiophanate-methyl resistance.

蒲占湑. 浙江地区柑桔炭疽病对甲基硫菌灵的抗性水平及其分子机制[J]. 中国农学通报, 2015, 31(17): 90-94.

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浙江地区柑桔炭疽病对甲基硫菌灵的抗性水平及其分子机制

Resistance Level and Mechanism of Colletotrichum gloeosporioides to Thiophanate-Methyl in Zhejiang

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