欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (35): 84-90.doi: 10.11924/j.issn.1000-6850.casb15080020

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

纳豆芽孢杆菌固体发酵条件及其优化研究

谭铭胜,厉大伟,邓元元,彭涵阁,兰时乐   

  1. 湖南农业大学生物科学技术学院,湖南农业大学生物科学技术学院,湖南农业大学生物科学技术学院,湖南农业大学生物科学技术学院,湖南农业大学生物科学技术学院
  • 收稿日期:2015-08-04 修回日期:2015-09-07 接受日期:2015-09-18 出版日期:2015-12-18 发布日期:2015-12-18
  • 通讯作者: 兰时乐
  • 基金资助:
    湖南省农业科技成果转化资金项目(2013NK4004)。

Studies on solid-state fermentation conditions and its optimization of Bacillus natto

  • Received:2015-08-04 Revised:2015-09-07 Accepted:2015-09-18 Online:2015-12-18 Published:2015-12-18

PDF (PC)

435

摘要： 为了优化纳豆芽孢杆菌固态发酵条件及确定适宜固态发酵培养基,以芽孢数为指标,采用固体发酵方法,研究了培养基组成、固液比、接种量、pH、培养基装量和发酵时间等发酵条件对芽孢数的影响,并通过响应面分析法确定了纳豆芽孢杆菌固态发酵的最佳发酵条件。结果表明：适宜的发酵条件为：麦麸58%、沸石粉34%、豆饼粉5.35%、葡萄糖2%、KH2PO40.5%、MgSO4?7H2O0.15%、pH 7.56、固液比1:1.1、装量50.39g/250mL三角瓶、接种量4.16%、发酵时间4d。在此条件下,芽孢数达到6.48×1010cfu/g。

关键词: 农业技术, 农业技术, 垦区农户, 技术需求, Logistic

Abstract: The study aims to optimize the solid-fermentation conditions of Bacillus natto and determine the suitable solid- fermentation medium composition. The spore number was used as index. Effects of the fermentation conditions, such as a medium component, the ratio of substance to water, inoculation, initial pH value, medium loading, cultural time and so on, on spore number were studied by using solid- state fermentation method, and the optimal fermentation condition of Bacillus natto solid fermentation was determined by using response surface analysis. The results showed that the optimal fermentation conditions were as follows: wheat bran 58%, zeolite powder 34%, soybean cake powder 5.35%, glucose 2%, KH2PO4 0.5%, MgSO4 ·7H2O 0.15%, pH 7.56, the ratio of substance to water 1:1.1, 50.39 g/250 mL triangle flask, inoculation 4.16%, fermentation time 4 d. Under these conditions, the number of spore reached 6.48×1010 CFU/g.