融合蛋白ABF 对金葡菌感染版纳微型猪近交系皮肤创伤的修复作用

doi:10.11924/j.issn.1000-6850.casb15090029

中国农学通报 ›› 2015, Vol. 31 ›› Issue (35): 27-33.doi: 10.11924/j.issn.1000-6850.casb15090029

所属专题：生物技术；畜牧兽医

融合蛋白ABF 对金葡菌感染版纳微型猪近交系皮肤创伤的修复作用

潘伟荣,李俊虹,肖晶,刘海京,刘欢,曾养志,查星琴,张兴举

云南省版纳微型猪近交系重点实验室昆明,云南省第一人民医院、昆明理工大学附属医院检验科,云南省版纳微型猪近交系重点实验室昆明,云南省版纳微型猪近交系重点实验室昆明,深圳华大基因研究院,云南省版纳微型猪近交系重点实验室昆明,云南省版纳微型猪近交系重点实验室昆明,深圳华大基因研究院，深圳华大方舟生物技术有限公司

收稿日期:2015-09-08 修回日期:2015-10-21 接受日期:2015-10-23 出版日期:2015-12-18 发布日期:2015-12-18
通讯作者: 张兴举
基金资助:
深圳市协同创新科技计划深港创新圈项目“金黄色葡萄球菌亚单位疫苗的开发”(SGLH20120928140607107)；深圳市知识创新计划基础研究项目“基于Cas9-guid RNA共表达系统构建乳腺特异性敲除BRCA1 基因小鼠的研究”(JCYJ20140717153620436)。

Protection Effect of Fusion Protein ABF Against Staphylococcus aureus in Banna Mini-pig Inbred Line Wound Infections

张兴举

Received:2015-09-08 Revised:2015-10-21 Accepted:2015-10-23 Online:2015-12-18 Published:2015-12-18

摘要/Abstract

摘要： 随着金葡菌对畜牧业破坏性增加和耐药性增强，人们对研发靶向金葡菌的有效保护性疫苗日趋迫切。为开发出更具免疫保护效力的抗菌疫苗，本研究首次利用EsxA、EsxB联合细菌鞭毛蛋白Flic，即制备出融合蛋白ABF，并在版纳微型猪近交系皮肤创伤模型上做实验验证。实验用版纳微型猪分为4组：对照组、模型组、实验组Ⅰ和实验组Ⅱ。结果发现单独使用EsxB蛋白与对照组微型猪的免疫指标之间差距并不显著，而预先经融合蛋白ABF免疫的微型猪，检测到其外周血免疫因子IgG 总量表达显著上升，即机体体液免疫水平提高，同时融合蛋白ABF 可以激活T 细胞。此外其脾脏淋巴细胞CD8a、CD3e 和IFN-γ阳性率均显著增高，皮肤创伤修复速度加快。总之，本实验研究成果证实融合蛋白ABF 可作为潜在有效的金葡菌亚单位疫苗，为防治金葡菌提供新的研究途径。

关键词: GAHP模型, GAHP模型, 渔业, 经济发展, 社会, 评估

Abstract: Staphylococcus aureus (SA), one of the major causes of livestock infection, is resistant to many kinds of antimicrobials. It is urgent to develop vaccines targeting at this pathogen. In this study, a fusion protein (ABF) composed of EsxA, EsxB and Flic was firstly generated. To examine the effect of ABF against SA, Banna mini-pig inbred line wound models were established and divided into four groups, the control wound group, the SA infected group, the EsxB pre-immunized group and the ABF pre-munized group. The results showed that peripheral IgG expression level in the mini-pig pre-immunized with ABF was significantly higher than that of the other three groups. Besides, ABF could activate T cell as well. The ratios of CD8a, CD3e and IFN-γ positive cells to spleen lymphocytes were all increased in the ABF group. In conclusion, all the results showed that ABF could significantly induce chemokine response and was functional during wound healing.

潘伟荣,李俊虹,肖晶,刘海京,刘欢,曾养志,查星琴,张兴举. 融合蛋白ABF 对金葡菌感染版纳微型猪近交系皮肤创伤的修复作用[J]. 中国农学通报, 2015, 31(35): 27-33.

张兴举. Protection Effect of Fusion Protein ABF Against Staphylococcus aureus in Banna Mini-pig Inbred Line Wound Infections[J]. Chinese Agricultural Science Bulletin, 2015, 31(35): 27-33.

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融合蛋白ABF 对金葡菌感染版纳微型猪近交系皮肤创伤的修复作用

Protection Effect of Fusion Protein ABF Against Staphylococcus aureus in Banna Mini-pig Inbred Line Wound Infections

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