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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (30): 12-16.doi: 10.11924/j.issn.1000-6850.casb16090055

所属专题： 玉米

• 农学 农业基础科学 • 上一篇    下一篇

电子束辐照玉米自交系M3的变异分析

黄强,苏鹏,吴茂力,任在斌   

  1. 四川省原子能研究院,四川省原子能研究院,四川省原子能研究院,四川省原子能研究院
  • 收稿日期:2016-09-12 修回日期:2017-10-17 接受日期:2016-11-25 出版日期:2017-10-31 发布日期:2017-10-31
  • 通讯作者: 黄强
  • 基金资助:
    四川省应用基础项目“电子束获取玉米突变体及遗传变异分析”(2011JY0131)；四川省科技创新工程“外来种质导入与辐射相结合创制高 配合力玉米新材料及其应用潜势分析”(2014ZZ0036)。

Variation Analysis of Maize Inbred Lines M3 by Electron Beam

  • Received:2016-09-12 Revised:2017-10-17 Accepted:2016-11-25 Online:2017-10-31 Published:2017-10-31

摘要： 为探讨电子束辐照对玉米自交系诱变效果，本研究分别利用剂量为200、300、400 Gy电子束对2份玉米自交系‘18-599’、‘K169’种子进行了辐照诱变，分别获得诱变株系(M3) 32 份、21 份，并对诱变株系和对照株系当代主要农艺性状进行了调查与分析。结果表明：2 份玉米自交系诱变M3株系株高与对照存在显著性差异；而穗位高与对照有差异显著的株系较少；主要雌穗性状诱变集中体现在穗长和穗行数，‘18-599’M3株系的穗长和穗行数与对照存在显著性差异；主要雄穗性状诱变效果因材料不同有差异；电子束辐照对油份和淀粉含量的改变效果较好，而2 份自交系诱变M3株系与对照的蛋白质含量均值均未达到显著水平。此研究结果表明，电子束辐照应用于玉米诱变育种可以引起较丰富的遗传变异，为利用电子束开展玉米自交系诱变育种以及玉米种质改良提供了依据。

关键词: 小麦种子, 小麦种子, POD活性, 变异, 育种

Abstract: Aiming at studying the mutagenic effects of electron beam irradiation on maize inbred lines, the seeds of two maize inbred lines‘18-599’and‘K169’were irradiated by electron beam with dose of 200, 300 and 400 Gy. Mutated strains (M3) were obtained from‘18-599’and‘K169’, which contained 32 mutants and 21 mutants respectively, and major agronomic characters of mutant strains as well as the wild type (control) were investigated in the contemporary generation. The results indicated that plant heights of two maize inbred M3 lines were significantly different from the wild type, while the number of ear height with significant difference was less. Mutagenic effects of electron beam irradiation on ear trait were mainly reflected in ear length and number of rows per ear, which showed significant difference between‘18-599’M3 lines and wild type, while mutagenic effects of electron beam irradiation on tassel traits were found to vary with different materials. Electron beam irradiation effects on the change of kernel oil and starch content was more obvious, but kernel protein content showed no significant difference between M3 lines and the wild type. In conclusion, the electron beam irradiation had great effects on morphological mutation in maize and this research could provide a basis for mutation breeding of maize by electron beam irradiation.