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中国农学通报 ›› 2017, Vol. 33 ›› Issue (32): 39-43.doi: 10.11924/j.issn.1000-6850.casb16110112

所属专题: 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

新疆野生欧洲李种子形态及组织结构研究

欧阳丽婷,耿文娟,冯贝贝,吕林杰,周媛   

  1. 新疆农业大学林园学院,新疆农业大学林园学院,新疆农业大学林园学院,新疆农业大学林园学院,新疆农业大学林园学院
  • 收稿日期:2016-11-22 修回日期:2017-11-06 接受日期:2017-01-24 出版日期:2017-11-27 发布日期:2017-11-27
  • 通讯作者: 耿文娟
  • 基金资助:
    新疆高校科研计划科学研究重点项目“新疆野生欧洲李种子生物学特性研究”(XJEDU2014I014);国家自然科学基金项目“野生欧洲李资源致濒因素及保育策略研究”(31560545);新疆维吾尔自治区园艺重点学科;新疆特色林果果实发育与调控创新团队资助项目。

Morphology and Structure of Wild European Plum Seed in Xinjiang

  • Received:2016-11-22 Revised:2017-11-06 Accepted:2017-01-24 Online:2017-11-27 Published:2017-11-27

摘要: 为掌握野生欧洲李种子特性,解决其繁殖困难的问题,以新疆野生欧洲李为研究对象,通过观测种子表型,并对其种壳、种皮及胚的石蜡切片显微结构及扫描电镜微观结构研究,掌握野生欧洲李种子形态及结构特点。研究结果显示:(1)新疆野生欧洲李种壳为椭圆形,两端略尖,浅棕色,具有坚硬的种壳,表面粗糙。种仁为扁平近卵形,种皮具纵向条纹。双子叶,白色。(2)扫描电镜下,种皮饰纹为形状不规则的褶起,多为近圆形、长椭圆形,又似蜂房状,并有排列不规则的瘤状突起;种壳表面饰纹呈现多层次不规则多边形连接状孔穴,连接边粗糙。(3)石蜡切片显示野生欧洲李种皮纵切由3 层构成,胚表层细胞近长方形,排列整齐紧密。野生欧洲李种子形态与其他李属植物种子的基本相似,但其微观形态在植物分类、亲缘关系研究中有重要研究价值。

关键词: 紫花苜蓿, 紫花苜蓿, 维生素, 高NaCl胁迫, 种子萌发及恢复

Abstract: To grasp the characteristics of wild European plum and solve the problems in its breeding, the authors took wild European plum growing in Xinjiang as the research object, based on the observation of seed phenotype, studied the microscopic structure and scanned electronic microscopic morphology of the shell and the paraffin sections of the seed coat and its embryo. Results showed that: (1) shell of the wild European plum was elliptic, slightly pointed at both ends, light brown, with a hard shell and rough surface; the kernel was flat nearly ovoid, dicotyledonous, white, and the seed coat of the wild European plum had vertical stripes; (2) under the electron microscope, the ornamentation of seed coat had irregularly shaped fold up, being suborbicular and long elliptic, honeycomb shaped and had arranged irregular nodular processes; the ornamentation of seed shell appeared multi-level irregular and polygon connecting holes with rough edges; (3) the paraffin sections of microscopic structure showed that seed coat of the wild European plum had three layers, the surface cells of the embryo were rectangular, arranged closely and neatly. The seed morphology of wild European plum is similar to that of other Prunus species, but its microscopic morphology has important research value in plant classification and genetic relationship research.

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