四川3种商业块菌可培养内生真菌的分离及鉴定

doi:10.11924/j.issn.1000-6850.casb17080125

中国农学通报 ›› 2018, Vol. 34 ›› Issue (24): 57-63.doi: 10.11924/j.issn.1000-6850.casb17080125

四川3种商业块菌可培养内生真菌的分离及鉴定

叶雷,付雨,邹捷,李小林

四川省农业科学院土壤肥料研究所,四川大学,四川农业大学,四川省农业科学院土壤肥料研究所

收稿日期:2017-08-29 修回日期:2018-08-16 接受日期:2017-10-24 出版日期:2018-08-28 发布日期:2018-08-28
通讯作者: 李小林
基金资助:
四川省科技支撑计划项目“突破性食用菌新品种选育与育种材料创新（块菌子课题）”(2016NYZ0040)；四川食用菌创新团队育种岗位资助 (川农业[2009]75 号)。

Isolation and Identification of Endophytic Fungi from Three Commercial Truffles in Sichuan Province

李小林

Received:2017-08-29 Revised:2018-08-16 Accepted:2017-10-24 Online:2018-08-28 Published:2018-08-28

摘要/Abstract

摘要： 旨在探究四川块菌主产区商业块菌的内生可培养真菌的群落结构及物种多样性。采集四川省凉山彝族自治州4个块菌产区（新田乡、新云乡、淌塘镇、雪山乡）的新鲜块菌子囊果作为实验材料，用马铃薯葡萄糖琼脂培养基（PDA培养基）对采集子囊果中的内生真菌进行分离培养，通过分子方法鉴定分离菌株的种属。从印度块菌（Tuber indicum）、假凹陷块菌(Tuber pseudoexcavatum )以及中华夏块菌(Tuber sinoaestivum)中共分离得到21株丝状真菌，分属于木霉属(Trichoderma)、毛壳属(Chaetomium)、青霉菌属(Penicillium)、镰刀菌属(Fusarium)以及链格孢属(Alternaria)，木霉属、镰刀菌属以及青霉菌属在块菌的子囊果中分布较丰富。初步鉴定出四川三种商业块菌的内生可培养真菌的种类，可能对块菌的储存保鲜、指导菌根化苗培育、探究块菌的发生机理机制都有重要意义，但对于具体原理还需要进一步探究。

关键词: 水产品, 水产品, 冻藏, 抗冻剂, 冷冻变性, 抗冻效果

Abstract: The aim of this study was to explore the culturable endophytic fungi community structure and diversity of the commercial truffles from the main producing areas in Sichuan. Fresh truffles were collected from 4 producing plots (Xintian, Xinyun, Tangtang and Xueshan) as experimental materials. PDA medias were used to isolation and culture the endophytic fungi in fruit, then identification the species of isolated by molecular method. Consequently, a total of 21 fungal were separated from Tuber indicum, Tuber pseudoexcavatum and Tuber sinoaestivum, including Trichoderma, Chaetomium, Penicillium, Fusarium and Alternaria. Among them, Trichoderma, Fusarium and Penicillium were the genera with higher abundances. Moreover, the study identification the type of culturable endophytic fungi preliminary from 3 kinds of commercial truffles in Sichuan province , it would greatly contribute to truffle preservation, to cultivation of mycorrhizal seedlings and to mechanism of truffle occurrence. However, the specific principles need to be further explored.

中图分类号:

叶雷,付雨,邹捷,李小林. 四川3种商业块菌可培养内生真菌的分离及鉴定[J]. 中国农学通报, 2018, 34(24): 57-63.

李小林. Isolation and Identification of Endophytic Fungi from Three Commercial Truffles in Sichuan Province[J]. Chinese Agricultural Science Bulletin, 2018, 34(24): 57-63.

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四川3种商业块菌可培养内生真菌的分离及鉴定

Isolation and Identification of Endophytic Fungi from Three Commercial Truffles in Sichuan Province

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