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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (5): 7-11.doi: 10.11924/j.issn.1000-6850.casb17120059

所属专题： 生物技术； 小麦

• 农学 农业基础科学 • 上一篇    下一篇

海水胁迫对黑小麦76种子萌发及幼苗期生理生化指标的影响

修妤, 石瑞常, 梁晓艳, 娄齐年, 顾寅钰, 王向誉, 郭光   

  1. 山东省农科院蚕业研究所
  • 收稿日期:2017-12-13 修回日期:2018-01-24 接受日期:2018-02-24 出版日期:2019-02-13 发布日期:2019-02-13
  • 通讯作者: 修妤
  • 基金资助:
    山东省农业科学院农业科技创新工程“海水农业关键技术”(CXGC2016B10)。

Seawater Stress on Seed Germination and Physiological-Biochemical Index in Seedling Stage of‘Black Wheat 76’

  • Received:2017-12-13 Revised:2018-01-24 Accepted:2018-02-24 Online:2019-02-13 Published:2019-02-13

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摘要： 为研究黑小麦76对海水的耐受能力,添加稀释后不同浓度的海水溶液处理小麦种子,5d后调查黑小麦种子萌发和幼苗生长情况。实验结果表明：5％、 10％、20％海水对小麦种子的萌发有轻微促进作用,在30％～40％海水浓度范围内,随海水浓度升高,种子的发芽率、发芽势、发芽指数呈现下降趋势。随着海水处理浓度的升高,苗长、根长、苗鲜重、根鲜重均呈现先增加后减少的趋势,表明黑小麦有较高的耐盐性,而且在海水胁迫下调控种子萌发,苗长、根长,苗鲜重、根鲜重各有一套机制,还有待于进一步研究。对叶片中SOD、POD、MDA、CAT活性和叶绿素含量进行测定,结果表明：随着海水浓度的增加,SOD值呈现先增加后降低的趋势,在浓度为10﹪时达到最大值,当盐浓度大于10﹪时,急剧下降；POD活性显著增加,到达30﹪时,POD活性达到最大值,随后随着海水浓度的增加,POD的活性呈现下降的趋势；MDA呈现逐渐增加的趋势；CAT活性呈现先增加后降低的趋势,在海水浓度为5﹪时达到峰值,后逐渐降低；叶绿素含量呈现逐渐降低的趋势。

关键词: 植棉土壤, 植棉土壤, 土层盐分, 相关性分析, 渭-库河绿洲

Abstract: To study the effect of seawater on‘black wheat 76’, we utilize seawater of different concentrations to treat the seed of‘black wheat 76’, and investigate the situation of seed germination and seedling growth after 5 days. The results indicated that seawater concentration of 5% , 10% and 20% could promote seed germination lightly, when the concentration was between 30% and 40%, the seed germination percentage, germinative force and germination index appeared a downtrend with the increase of the concentration. Seedling length, root length, seedling fresh weight and root fresh weight increased and then decreased with the increase of seawater concentration, and there were respective regulation mechanisms to control seedling length, root length, seedling fresh weight and root fresh weight, which needed further study. The activity of SOD、POD、 CAT and the content of MDA and chlorophyll in the leaf were measured, and the results showed that the activity of SOD increased firstly and then decreased with the increase of seawater concentration, and reached the highest when the concentration was 10%, and decreased sharply when the concentration was higher than 10% ; the activity of POD increased sharply, and reached the highest at concentration of 30% , and then decreased; the activity of CAT increased firstly and then decreased, reached the peak at the concentration of 5% , then decreased gradually; the MDA content assumed a trend of increasing; the chlorophyll content assumed a trend of decreasing gradually.