不同水稻品种OsVDAC3及3个互作蛋白基因OsV3IP1-3 表达模式分析

doi:10.11924/j.issn.1000-6850.casb18100075

中国农学通报 ›› 2019, Vol. 35 ›› Issue (6): 101-107.doi: 10.11924/j.issn.1000-6850.casb18100075

所属专题：生物技术；水稻

不同水稻品种OsVDAC3及3个互作蛋白基因OsV3IP1-3 表达模式分析

王亚楠, 程钢, 覃永华, 谭艳平, 刘学群, 徐鑫, 刘新琼, 王春台

中南民族大学大学生命科学学院

收稿日期:2018-10-22 修回日期:2019-01-19 接受日期:2018-12-19 出版日期:2019-02-22 发布日期:2019-02-22
通讯作者: 王春台
基金资助:
国家自然科学基金项目“水稻VDACs互作蛋白的筛选及作用机理研究”(31170226)；湖北重点实验室建设基金(2018BFC360)。

OsVDAC3 and 3 Interaction Protein Genes OsV3IP1-3 in Rice Cultivars: Expression Pattern Analysis

Received:2018-10-22 Revised:2019-01-19 Accepted:2018-12-19 Online:2019-02-22 Published:2019-02-22

摘要/Abstract

摘要： 为研究OsVDAC3 及互作蛋白基因OsV3IP1-3 的表达模式，用生物信息学和定量PCR方法分析了水稻不育系‘粤泰A’(YTA)、保持系‘粤泰B’(YTB)和‘日本晴’(NIP)不同发育时期幼穗，四叶期根、鞘和叶中OsVDAC3 和OsV3IP1-3 的表达量。生物信息学分析表明在ATG上游含有21 个与水稻生长发育及胁迫应答相关的顺式作用元件；RiceXPro 和MSU Rice Genome Annotation Project 水稻基因表达数据库分析结果表明在NIP 中OsV3IP1 只在幼苗表达，而OsVDAC3、OsV3IP2 和OsV3IP3 在整个生活周期都表达，但OsVDAC3 在花药表达水平最高，OsV3IP2 在生殖器官表达水平最高，OsV3IP3 在嫩芽中表达量最高。定量PCR结果表明OsVDAC3 和OsV3IP1-3 基因在NIP、YTA和YTB 3 个水稻品种中表达模式不同。OsVDAC3 在YTB的1.5~4.0 cm幼穗表达水平非常高，OsV3IP1 在3 个品种的四叶期地上部都有非常高的表达，OsV3IP2 和OsV3IP3 在YTB和NIP 中表达模式相似，而与YTA中差异明显。这些基因的表达模式与功能的关系有待进一步研究。

关键词: 春玉米, 春玉米, CERES-Maize, 遗传参数, 吉林

Abstract: To study the expression patterns of OsVDAC3 and its interaction protein gene OsV3IP1-3 , the expression ofOsVDAC3 andOsV3IP1-3 in the inflorescences of different developmental stages and the root, sheath and leaf at the fourth-leaf stage from the rice sterile line‘Yuetai A’(YTA), the maintenance line ‘Yuetai B’(YTB) and‘Nipponbare’(NIP) were analyzed with bioinformatics and RT-PCR. The results of bioinformatics analysis showed that there were 21 Cis- action elements related with the growth and stress response of rice in the upstream of ATG. The analysis results of rice gene expression database from RiceXPro and MSU Rice Genome Annotation Project showed that OsV3IP1 in Nipponbare was only expressed in the seedlings, but OsVDAC3 , OSV3IP2 and OSV3IP3 were expressed throughout the life cycle, furthermore OsVDAC3 had the highest expression in anther, OSV3IP2 had the highest expression in the reproductive organs, and OSV3IP3 had the highest expression in the buds. The results of RT- PCR showed that the expression patterns ofOsVDAC3 andOsV3IP1-3 were different in NIP, YTA and YTB. The expression level ofOsVDAC3 in young panicles (1.5-4.0 cm) of YTB was very high.OsV3IP1 had very high expression at the fourth-leaf stage among the 3 cultivars, the expression patterns ofOsV3IP2 andOsV3IP3 in YTB and NIP were similar, but were different from that in YTA. The relationship between the expression patterns and functions of these genes needs further study.

中图分类号:

王亚楠,程钢,覃永华,谭艳平,刘学群,徐鑫,刘新琼,王春台. 不同水稻品种OsVDAC3及3个互作蛋白基因OsV3IP1-3 表达模式分析[J]. 中国农学通报, 2019, 35(6): 101-107.

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不同水稻品种OsVDAC3及3个互作蛋白基因OsV3IP1-3 表达模式分析

OsVDAC3 and 3 Interaction Protein Genes OsV3IP1-3 in Rice Cultivars: Expression Pattern Analysis

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