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中国农学通报 ›› 2019, Vol. 35 ›› Issue (24): 46-51.doi: 10.11924/j.issn.1000-6850.casb19030030

所属专题: 生物技术 园艺 农业生态

• 林学 园艺 园林 • 上一篇    下一篇

外源亚精胺对低温冷害下菜豆种子萌发及抗性的影响

刘畅, 刘大军, 闫志山, 范有君, 杨晓旭, 冯国军   

  1. 黑龙江大学
  • 收稿日期:2019-03-08 修回日期:2019-04-03 接受日期:2019-04-24 出版日期:2019-08-26 发布日期:2019-08-26
  • 通讯作者: 冯国军
  • 基金资助:
    黑龙江省省属高等学校基本科研业务费基础研究项目“多胺缓解菜豆低温胁迫的作用机制”(KJCXYB201705)。

Exogenous Spd: Effects on Germination and Resistance of Snap Bean Seeds Under Low Temperature Stress

  • Received:2019-03-08 Revised:2019-04-03 Accepted:2019-04-24 Online:2019-08-26 Published:2019-08-26

摘要: 为了探讨外源亚精胺对低温条件下菜豆种子萌发与种子耐低温性的影响。本研究以菜豆品种‘热那亚’为试验材料,设置常温对照(CK)、4℃低温(LT)以及4℃低温条件下添加1 mmol/L Spd (LT+Spd)三个处理组,对其发芽率、发芽指数、种子活力及生理指标进行测定。结果显示:4℃低温条件下,添加1 mmol/L的Spd后菜豆种子发芽率、发芽指数和种子活力分别提高了95.69%、136.10%和217.12%;电解质外渗率降低;抗氧化酶SOD、POD、CAT的活性升高;H2O2、O2-、MDA、脯氨酸和可溶性蛋白的含量降低。由此可见,施用外源亚精胺能够缓解低温胁迫对菜豆种子造成的伤害,提高菜豆种子的耐低温性,从而提高菜豆种子的发芽率、发芽指数和种子活力。

关键词: 高粱, 高粱, 再生高粱, 泸糯12号, 栽培技术, 留桩高度

Abstract: In order to study the effect of exogenous Spd on seed germination and tolerance of snap bean under low temperature. In this study, the snap bean cultivar ‘Renaya’ was used as experimental material. Three treatments, normothermia control (CK), 4℃ low temperature treatment (LT) and addition of 1mmol/L Spd at 4℃ (LT+Spd) were used to determined germination rate, germination index, seed vigor and physiological index. The results showed that germination rate, germination index and seed vigor increased by 95.69%, 136.10% and 217.12%, respectively, after adding 1mmol/L Spd at 4℃; the electrolyte leakage rate decreased, the activities of antioxidant enzymes SOD, POD and CAT increased, and the contents of H2O2, O2-, MDA, proline and soluble protein decreased. Therefore, the application of exogenous Spd can alleviate the damage caused by low temperature stress on snap bean seeds, improve the low temperature tolerance, thereupon then improving the germination rate, germination index and seed vigor of snap bean seeds.