荧光假单胞菌Pseudomonas fluorescens RN-88抑菌特性研究

doi:10.11924/j.issn.1000-6850.casb2023-0310

中国农学通报 ›› 2024, Vol. 40 ›› Issue (11): 22-27.doi: 10.11924/j.issn.1000-6850.casb2023-0310

荧光假单胞菌Pseudomonas fluorescens RN-88抑菌特性研究

朱李霞¹^,²(), 周蓉蓉³, 张赛男¹, 毛洪刚¹^,², 夏禧龙¹, 李春雨¹, 尹冬敏¹, 任建军¹()

¹ 常州大学城乡矿山研究院，生物质高效炼制及高质化利用国家地方联合工程中心，常州市生物质绿色安全高值利用技术重点实验室，江苏常州 213164
² 常州大学药学院，生物与食品工程学院，江苏常州 213164
³ 北京市畜牧总站，北京 100107

收稿日期:2023-04-27 修回日期:2023-07-15 出版日期:2024-04-15 发布日期:2024-04-11
通讯作者:
任建军，男，1983年出生，山西朔州人，研究员，博士，研究方向：农业与环保微生物技术。通信地址：213164 江苏省常州市武进区滆湖中路21号，Tel：0519-86330101，E-mail：rjj@cczu.edu.cn。
作者简介:
朱李霞，女，2000年出生，江苏南通人，硕士在读，研究方向：农业与环保微生物技术。通信地址：213164 江苏省常州市武进区滆湖中路21号，Tel：0513-86330101，E-mail：s22090860035@smail.cczu.edu.cn。
基金资助:
贵州省科技计划项目“养殖废弃物无害化处理与资源化利用技术集成应用研究”(黔科合支撑【2022】一般175); “江苏省高等学校基础科学（自然科学）研究重大项目”(23KJA610001); 北京市家禽创新团队(BAIC062023)

Antifungal Characters of Pseudomonas fluorescens RN-88

ZHU Lixia¹^,²(), ZHOU Rongrong³, ZHANG Sainan¹, MAO Honggang¹^,², XIA Xilong¹, LI Chunyu¹, YIN Dongmin¹, REN Jianjun¹()

¹ Changzhou Key Laboratory of Biomass Green, Safe and High Value Utilization Technology/ National-Local Joint EngineeringResearch Center of Biomass Refining and High-Quality Utilization/ Institute of Urban and Rural Mining, Changzhou University, Changzhou, Jiangsu 213164
² School of Pharmacy/ School of Biological and Food Engineering, Changzhou University, Changzhou, Jiangsu 213164
³ Beijing General Station of Animal Husbandry, Beijing 100107

Received:2023-04-27 Revised:2023-07-15 Published:2024-04-15 Online:2024-04-11

摘要/Abstract

摘要：

本研究旨在利用拮抗菌防治植物病害，为桃的病害生物防治提供更多可行性的方案。以前期筛选获得的一株桃褐腐病拮抗细菌荧光假单胞菌(Pseudomonas fluorescens)RN-88为试验材料，采用杯碟法测定发酵液对桃褐腐菌(Monilinia fructicol)的抑菌活性，并结合抑菌圈法，研究了该菌在pH、温度、紫外线(UV)等不同环境中的稳定性。实验结果表明，细菌RN-88能显著降低桃褐腐病的发病率，其发酵液对褐腐病菌(M. fructicol)的抑制率可达44.69%。在过滤除菌后，相对于高温灭菌，发酵液中抑菌物质的损失较小。在pH 7.5~9.0的碱性溶液中，发酵液的抑菌活性有较好的稳定性，但在酸性溶液(3.0≤pH≤6.5)中处理后，其抑菌能力减弱。发酵液的抑菌率几乎不受温度和紫外线(UV)的影响。Pseudomonas fluorescens RN-88细菌具有较好的环境稳定性，具有开发成桃褐腐病生物拮抗剂的潜在价值，本研究为荧光假单胞菌作为新型生防菌的应用提供了理论依据，同时为实现桃病害的生物防治提供了更多可行性的方案。

关键词: 桃褐腐病, 生防细菌资源, 抑菌活性, 环境稳定性, 荧光假单胞菌

Abstract:

This study aims to use antagonistic bacteria to control plant diseases, and provide more feasible biological control methods for the diseases control of peach. A strain Pseudomonas fluorescens RN-88 obtained previously in the authors lab was used to determine the inhibitory activity of the fermentation broth against Monilinia fructicol, which was determined by the cup and saucer method. The stability of the broth under different environments such as pH, temperature, and ultraviolet (UV) light was evaluated by the inhibition circle method. The results showed that the antagonistic bacterium RN-88 reduced the incidence of brown rot in peaches, and its fermentation broth inhibited M. fructicol up to 44.69%, and the loss of fungal inhibitory ability of the fermentation broth was effectively reduced by filtration compared to autoclaving. The inhibition activity of the fermentation broth was stable in alkaline solutions at pH 7.5 - 9.0, and was reduced in acidic solutions (3.0 ≤ pH ≤ 6.5). There was no effect on the inhibition rate of the fermentation broth by temperature and ultraviolet (UV) light. All above, fermentation broth of Pseudomonas fluorescens RN-88 has good environmental stability which has the potential to be developed to a novel fungicide. This study also provides a theoretical basis for the application of Pseudomonas fluorescens.

Key words: Monilinia fructicola, biocontrol bacteria resources, inhibition activity, environmental tolerance, Pseudomonas fluorescens

朱李霞, 周蓉蓉, 张赛男, 毛洪刚, 夏禧龙, 李春雨, 尹冬敏, 任建军. 荧光假单胞菌Pseudomonas fluorescens RN-88抑菌特性研究[J]. 中国农学通报, 2024, 40(11): 22-27.

ZHU Lixia, ZHOU Rongrong, ZHANG Sainan, MAO Honggang, XIA Xilong, LI Chunyu, YIN Dongmin, REN Jianjun. Antifungal Characters of Pseudomonas fluorescens RN-88[J]. Chinese Agricultural Science Bulletin, 2024, 40(11): 22-27.

图/表 5

处理	真菌菌落直径/mm	抑菌率/%
对照	46.75 ± 0.33	-
滤膜除菌	25.50 ± 5.69	44.69 (a)
高温灭菌	34.52 ± 5.78	25.96 (b)

表1. 不同灭菌处理后发酵液的抑菌率

图1. 不同pH下发酵液抑菌活性的稳定性误差线上方不同的小写字母表示处理间差异显著(P<0.05)

图2. 不同温度下发酵液抑菌活性的稳定性

UV处理时间/min	真菌菌落直径/mm
对照	40.90 ± 0.67 (ab)
15	41.63 ± 1.64 (a)
30	45.72 ± 2.39 (a)
60	43.00 ± 0.23 (a)
120	45.90 ± 8.35 (a)

表2. 紫外线处理后发酵液的抑菌率

图3. UV处理后发酵液的抑菌能力图中左边皿为对照（添加原发酵液），右边皿为处理试验（添加紫外照射后的发酵液）

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荧光假单胞菌Pseudomonas fluorescens RN-88抑菌特性研究

Antifungal Characters of Pseudomonas fluorescens RN-88

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