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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (12): 59-64.doi: 10.11924/j.issn.1000-6850.casb2023-0478

所属专题： 土壤重金属污染

• 资源·环境·生态·土壤 • 上一篇    下一篇

微生物电化学修复中污染土壤生化特性变化

秦宵睿1,2(), 乔青青1,2, 景秀清1,2, 翟飞红1,2, 赵晓东1,2,3(), 李晓晶3, 李永涛4   

  1. 1 太原师范学院生物科学与技术学院，山西榆次 030619
    2 太原师范学院，汾河流域地表过程与资源生态安全山西省重点实验室，山西榆次 030619
    3 农业农村部环境保护科研监测所，农业农村部产地环境污染防控重点实验室，天津市农业环境与农产品安全重点实验室，天津 300191
    4 华南农业大学资源环境学院，广州 510642
  • 收稿日期:2023-06-24 修回日期:2023-09-09 出版日期:2024-04-25 发布日期:2024-04-22
  • 通讯作者:
    赵晓东，男，1990年出生，山西长治人，副教授，博士，研究方向：土壤有机污染修复。通信地址：030619 山西省晋中市榆次区大学街319号 太原师范学院，E-mail：。
  • 作者简介:

    秦宵睿，女，1998年出生，山西长治人，硕士，研究方向：土壤污染生物修复。通信地址：030619 山西省晋中市榆次区大学街319号 太原师范学院，E-mail：。

  • 基金资助:
    国家自然科学基金资助项目“土壤微生物电化学-基因工程菌联合促进四环素降解的机理研究”(42007121); “铁介导石油烃污染土壤生物电化学修复机理研究”(41977133); 国家重点研发计划“基于土壤生物与生物电流强化的抗生素/激素生物降解技术研发”(2017YFD0800704); 山西省高等学校科技创新项目“四环素在土壤微生物电化学系统阴极的降解机理研究”(2020L0536)

Changes of Soil Biochemical Characteristics in Microbial Electrochemical Remediation of Contaminated Soil

QIN Xiaorui1,2(), QIAO Qingqing1,2, JING Xiuqing1,2, ZHAI Feihong1,2, ZHAO Xiaodong1,2,3(), LI Xiaojing3, LI Yongtao4   

  1. 1 College of Biological Sciences and Technology, Taiyuan Normal University, Yuci, Shanxi 030619
    2 Shanxi Key Laboratory of Earth Surface Processes and Resource Ecology Security in Fenhe River Basin, Taiyuan Normal University, Yuci, Shanxi 030619
    3 Agro-environmental Protection Institute, Ministry of Agriculture and Rural Affairs (MARA)/Key Laboratory of Original Agro-environmental Pollution Prevention and Control of MARA/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Tianjin 300191
    4 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642
  • Received:2023-06-24 Revised:2023-09-09 Published:2024-04-25 Online:2024-04-22

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摘要：

为探明土壤酶和pH在微生物电化学系统(MES)降解四环素过程中的作用，采集了黑土、潮土、黄棕壤和红壤4种类型土壤进行研究。结果表明：向黑土、潮土和红壤中添加10 mg/kg四环素后（TN处理）土壤pH较对照处理（CK，无抗生素添加）分别增加了0.08、0.15、1.09个单位，而经MES处理后（TC，四环素含量10 mg/kg）4种类型土壤中pH均呈降低趋势。尽管土壤脱氢酶、脲酶和多酚氧化酶活性在四环素污染土壤中展现出不同的变化规律，但其活性在MES中较TN处理分别增加了62.4%~72.1%、11.3%~80.9%（红壤除外）和12.6%~42.6%。与TN相比，四环素降解率在TC处理中分别增加了14%（黑土）、26%（潮土）、26%（黄棕壤）和17%（红壤）。相关性分析表明，脱氢酶活性与四环素降解率呈显著正相关，pH与降解率显著负相关，说明脱氢酶可能参与了降解四环素过程，且酸性条件可能更有利于降解。该试验从土壤生化特性角度揭示了MES对四环素的降解机理，可为土壤污染修复提供支撑。

关键词: 微生物电化学系统, 不同类型土壤, 四环素, pH, 酶活性

Abstract:

To explore the role of soil enzymes and pH in the degradation of tetracycline in Microbial Electrochemical Systems (MES), four types of soils including black soil, moisture soil, yellow brown soil and red soil were collected for study. The results showed that after adding tetracycline (TN treatment, 10 mg/kg) to black soil, moisture soil and red soil, the values of pH increased by 0.08, 0.15 and 1.09 units respectively compared with the control treatment (CK, without tetracycline addition). However, in MES treatment (TC, tetracycline concentration 10 mg/kg), the values of pH in the four types of soil all showed downward trend. Although the soil dehydrogenase, urease and polyphenol oxidase activities exhibited different variation trends in tetracycline contaminated soil, their activities in MES increased by 62.4%-72.1%, 11.3%-80.9% (except red soil) and 12.6%-42.6% compared with TN treatment, respectively. Meanwhile, the degradation rate of tetracycline in TC treatment was respectively increased by 14% (black soil), 26% (moisture soil), 26% (yellow brown soil) and 17% (red soil) compared with TN treatment. Correlation analysis indicated that dehydrogenase activity showed significant positive correlation with tetracycline degradation rate, while pH showed significant negative correlation with degradation rate, indicating that dehydrogenase might participate in the process of tetracycline degradation, and acidic conditions may be more conducive to degradation. This experiment revealed the degradation mechanism of tetracycline by MES based on soil biochemical characteristics, which could provide important support for soil pollution remediation.

Key words: microbial electrochemical system, different types of soil, tetracycline, pH, enzyme activity