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中国农学通报 ›› 2024, Vol. 40 ›› Issue (18): 22-30.doi: 10.11924/j.issn.1000-6850.casb2023-0505

• 农学·农业基础科学 • 上一篇    下一篇

132份甘薯地方种质遗传多样性和群体结构分析

陈伊航1,2(), 唐朝臣2, 张荣2, 姚祝芳2, 金晶炜1(), 王章英2()   

  1. 1 西北农林科技大学草业与草原学院,陕西杨陵 712100
    2 广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室,广州 510640
  • 收稿日期:2023-07-28 修回日期:2023-10-16 出版日期:2024-06-25 发布日期:2024-06-18
  • 通讯作者:
    金晶炜,女,1980年出生,内蒙古扎兰屯人,副研究员,博士,研究方向:半干旱区草地植被恢复与重建。通信地址:712100 陕西省咸阳市杨陵区邰城路3号,Tel:18291994289,E-mail:
    王章英,女,1978年出生,河北衡水人,研究员,博士,研究方向:甘薯资源与遗传育种。通信地址:510640 广东省广州市天河区金颖西二街18号,Tel:15817096369,E-mail:
  • 作者简介:

    陈伊航,男,1998年出生,湖南娄底人,硕士研究生,研究方向:甘薯种质资源。通信地址:510640 广东省广州市天河区金颖西二街18号,Tel:17612918779,E-mail:

  • 基金资助:
    作物种质资源保护与利用专项“甘薯种质资源安全保存”(NWB014); 国家作物种质资源库“甘薯广州分库运行分库”(NCGRC-2023-39); 现代农业产业技术体系 “国家甘薯产业技术体系”(CARS-10)

132 Sweetpotato Landraces: Genetic Diversity and Population Structure

CHEN Yihang1,2(), TANG Chaochen2, ZHANG Rong2, YAO Zhufang2, JIN Jingwei1(), WANG Zhangying2()   

  1. 1 College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi 712100
    2 Crops Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Guangzhou 510640
  • Received:2023-07-28 Revised:2023-10-16 Published:2024-06-25 Online:2024-06-18

摘要:

本研究旨在评估中国甘薯地方种质资源的遗传多样性和群体结构。研究以132份甘薯地方种质为材料,对其表型性状、类胡萝卜素含量以及SSR分子标记进行遗传多样性分析和群体结构分析。分析结果表明,132份甘薯地方种质表型性状的平均变异系数为45.84%,类胡萝卜素含量平均为6.33 μg/g,最高为59.90 μg/g,SSR分子标记的Nei’s多样性指数平均为0.15、Shannon’s多样性指数平均为0.23,表明存在一定程度的遗传多态性。进一步的群体结构分析表明,这132份甘薯地方种质可分为5个亚群。分子变异分析(AMOVA)显示,84%的变异存在于群体内,16%的变异存在于群体间。综上所述,中国的甘薯地方种质具有丰富的遗传多样性,且种质之间的遗传变异主要集中在亚群内部。这对于中国甘薯地方种资源的保护和新品种的培育提供了重要的信息。

关键词: 甘薯, 表型性状, SSR分子标记, 类胡萝卜素含量, 遗传多样性, 群体结构, 地方种质

Abstract:

Understanding the genetic diversity and population structure of sweetpotato landraces in China is helpful for the protection of sweetpotato germplasm resources and the cultivation of new varieties. In this study, 132 sweet potato landraces were used as materials to analyze the genetic diversity and population structure of phenotypic traits, carotenoid content, and SSR molecular markers. The results showed that the average coefficient of variation of phenotypic traits of 132 sweetpotato landraces was 45.84%, the average carotenoid content was 6.33 μg/g, and the highest was 59.90 μg/g. The average Nei's diversity index of SSR molecular markers was 0.15, and the average Shannon's diversity index was 0.23. Based on the population structure analysis, the 132 sweetpotato landraces were divided into five subpopulations. Further analysis of molecular variation revealed that 84% of the variation existed within populations and 16% between populations. In summary, sweetpotato landraces have rich genetic diversity, and the genetic variation among germplasms is mainly concentrated within the subpopulation. This provides important information for the protection of local sweetpotato germplasm resources and the cultivation of new varieties in China.

Key words: sweetpotato, phenotypic traits, SSR markers, carotenoid content, genetic diversity, population structure, local germplasm