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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (12): 94-99.doi: 10.11924/j.issn.1000-6850.casb2024-0343

• 生物科学 • 上一篇    下一篇

陕西番茄和辣椒番茄斑萎病毒的分子鉴定

刘晨1,2(), 杨艺炜1,2, 魏佩瑶1,2, 潘嵩1,2, 陈志杰1,2, 李英梅1,2()   

  1. 1 陕西省生物农业研究所，西安 710043
    2 陕西省植物线虫学重点实验室，西安 710043
  • 收稿日期:2024-05-27 修回日期:2024-09-15 出版日期:2025-04-25 发布日期:2025-04-24
  • 通讯作者:
    李英梅，女，1975年出生，陕西长武人，研究员，硕士，研究方向：果蔬病虫害绿色防控。通信地址：710043 陕西省西安市新城区咸宁中路125号陕西省科学院后楼，Tel：029-82291059，E-mail：。
  • 作者简介:

    刘晨，女，1988年出生，陕西咸阳人，副研究员，硕士，研究方向：植物保护。通信地址：710043 陕西省西安市新城区咸宁中路125号陕西省科学院后楼，Tel：029-82291059，E-mail：。

  • 基金资助:
    咸阳市科技局重大科技创新专项“基于中医理论的番茄病毒病绿色防控关键技术研究与示范”(L2023-ZDKJ-CYJQ-NY-004)

Identification of Tomato Spotted Wilt Orthotospovirus on Tomato and Pepper in Shaanxi

LIU Chen1,2(), YANG Yiwei1,2, WEI Peiyao1,2, PAN Song1,2, CHEN Zhijie1,2, LI Yingmei1,2()   

  1. 1 Bio-Agriculture Institute of Shaanxi, Xi’an 710043
    2 Shaanxi Key Laboratory of Plant Nematology, Xi’an 710043
  • Received:2024-05-27 Revised:2024-09-15 Published:2025-04-25 Online:2025-04-24

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摘要： 为明确引起陕西番茄叶片褪绿坏死、果实出现坏死斑纹、同心圆斑以及辣椒叶片褐色枯萎、坏死、果实出现畸形、皱缩等现象的病毒种类，通过采集具有典型感病症状的番茄和辣椒组织，利用番茄斑萎病毒(Tomato spotted wilt virus, TSWV)特异性引物及N基因引物对样品进行RT-PCR的扩增检测。结果表明，所采集番茄和辣椒样品中均存在TSWV的感染。通过对N基因序列进行系统发育树构建，陕西番茄和辣椒分离的TSWV与澳大利亚辣椒、日本番茄及中国北京马鞭草分离的TSWV亲缘关系更近。病毒回接至健康的番茄苗和辣椒苗20 d后，番茄和辣椒均出现褪绿、枯萎、皱缩等症状，RT-PCR检测植株均携带TSWV。本研究证实陕西番茄和辣椒已受到TSWV的侵染为害，可为TSWV的防控提供理论依据。

关键词: 陕西, 番茄, 辣椒, 番茄斑萎病毒, 分子鉴定

Abstract:

In order to definite the species of virus, which cause tomato leaf samples chlorosis necrosis, fruit necrosis, and concentric spots, we collected the susceptible tomato samples, and pepper leaf samples exhibiting symptoms of brown wilting and necrosis, fruit deformity and shriveling. RT-PCR detection was performed utilizing specific primers targeting the tomato spotted wilt virus (TSWV) as well as N gene primers. The results indicated that occurrence of TSWV infection in both tomato and pepper samples. Phylogenetic analysis based on the N gene sequence demonstrated a close relationship between TSWV isolates obtained from tomatoes and peppers in Shaanxi with those found in Australian peppers, Japanese tomatoes, as well as Beijing Verbenas within China. During the virus inoculation experiment, tomato and pepper samples both displayed characteristic symptoms including chlorosis, necrotic foliage, and shriveled fruits; RT-PCR successfully detected the presence of TSWV in these plants. This study confirms that tomatoes and peppers in Shaanxi have been affected by TSWV infection and provides a theoretical basis for its prevention and control.

Key words: Shaanxi Province, tomato, pepper, tomato spotted wilt virus, molecular identification