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中国农学通报 ›› 2025, Vol. 41 ›› Issue (32): 116-123.doi: 10.11924/j.issn.1000-6850.casb2024-0734

所属专题: 畜牧兽医

• 畜牧·动物医学·蚕·蜂 • 上一篇    下一篇

内蒙古草原16种植物提取物抗氧化活性研究

赵志惠1,2(), 代继云1,2, 刘宇宁1,2, 付慧1,2, 盈盈1,2, 黄奕颖1,2, 降晓伟1,2, 刘江英1,2, 卫媛1,2, 张燕东1,2, 王丽霞1,2, 于海东1,2, 万玉萌1,2, 邱鹏程1,2, 石富1,2, 刘婷璐1,2, 孙凤舞1,2, 吴洪新1,2()   

  1. 1 中国农业科学院草原研究所/内蒙古自治区农畜产品质量安全中心,呼和浩特 010013
    2 鄂尔多斯农产品质量安全中心,鄂尔多斯 017000
  • 收稿日期:2024-12-10 修回日期:2025-02-15 出版日期:2025-11-18 发布日期:2025-11-18
  • 通讯作者:
    吴洪新,女,1978年出生,内蒙古赤峰人,研究员,硕士,研究方向:牧草及草畜产品品质评价。通信地址:010000 呼和浩特市赛罕区乌兰察布东街120号 中国农业科学院草原研究所,Tel:0471-4961330,E-mail:
  • 作者简介:

    赵志惠,女,1992年出生,内蒙古乌兰察布人,助理研究员,硕士,研究方向:草畜农产品质量安全检测。通信地址:010000 呼和浩特市赛罕区乌兰察布东街120号 中国农业科学院草原研究所,Tel:0471-4961330,E-mail:

  • 基金资助:
    内蒙古自治区自然科学基金青年基金项目“基于单克隆抗体的酶联免疫分析方法用于环境-苜蓿-畜产品中咪草烟快速检测”(2022QN03006); 中国农业科学院北方农牧业技术创新中心资助项目“优质农草畜产品品质大数据分析平台研建与内蒙古草原羊肉品质-产地智能溯源系统开发”(BFGJ2022006); 中国农业科学院北方农牧业技术创新中心资助项目“呼和浩特市肉羊精准化选育及良种繁育体系建设”(2023RC-产研院-6); 中央级公益性科研院所基本科研业务费专项“基于固相微萃取结合气质联用技术构建内蒙古不同品种草原羊肉挥发性风味物质指纹图谱”(1610332022004)

Study on Antioxidant Activity of Extracts from 16 Plants in Inner Mongolia Grassland

ZHAO Zhihui1,2(), DAI Jiyun1,2, LIU Yuning1,2, FU Hui1,2, YING Ying1,2, HUANG Yiying1,2, JIANG Xiaowei1,2, LIU Jiangying1,2, WEI Yuan1,2, ZHANG Yandong1,2, WANG Lixia1,2, YU Haidong1,2, WAN Yumeng1,2, QIU Pengcheng1,2, SHI Fu1,2, LIU Tinglu1,2, SUN Fengwu1,2, WU Hongxin1,2()   

  1. 1 Institute of Grassland Research, Chinese Academy of Agricultural Sciences/Inner Mongolia Autonomous Region Agricultural and Livestock Product Quality and Safety Center, Hohhot 010013
    2 Ordos Agricultural Product Quality and Safety Center, Ordos, Inner Mongolia 017000
  • Received:2024-12-10 Revised:2025-02-15 Published:2025-11-18 Online:2025-11-18

摘要:

为探究内蒙古草原植物提取物的抗氧化活性,本研究以达拉特旗及乌拉盖草原的16种植物为试验材料,采用福林酚法、三氯化铝比色法、浓硫酸-苯酚比色法及香草醛-高氯酸比色法,分别测定各提取物中总多酚、总黄酮、总多糖及总皂苷含量;同时以DPPH、ABTS自由基清除率和总抗氧化能力为指标,开展体外抗氧化活性评价。结果显示,16种植物提取物中,各组分含量及抗氧化活性差异明显,其中,地榆和叉分蓼中总多酚含量最高,达到33.22%和29.34%,叉分蓼中总黄酮含量最高,达到17.72%,且地榆和叉分蓼提取物的DPPH、ABTS自由基清除率和总抗氧化能力最强。本研究明确了内蒙古草原高抗氧化活性植物资源,为草原植物的抗氧化功能挖掘、医药及功能产品开发,以及草原植物资源的可持续利用提供了科学理论支撑。

关键词: 草原植物, 植物提取物, 总多酚, 总黄酮, 总多糖, 总皂苷, 抗氧化活性

Abstract:

To investigate the antioxidant activity of plant extracts from Inner Mongolia grassland, 16 grassland plant species collected from Dalate Banner and Ulgai grassland were used as experimental materials. The contents of total phenolic, total flavonoid, total polysaccharide, and total saponin in the extracts were determined using the Folin-Ciocalteu method, aluminum chloride colorimetric method, concentrated sulfuric acid-phenol colorimetric method, and vanillin-perchloric acid colorimetric method, respectively. Antioxidant activity was evaluated using DPPH and ABTS free radical scavenging rates along with the total antioxidant capacity as indicators. The results showed that significant differences were in the content of these component and antioxidant activity among the 16 plant extracts. Sanguisorba officinalis and Koenigia divaricata exhibited the highest total phenolic content, reaching 33.22% and 29.34%, respectively. Koenigia divaricata also had the highest total flavonoid content, at 17.72%. Moreover, the extracts of Sanguisorba officinalis and Koenigia divaricata exhibited the strongest DPPH and ABTS radical scavenging abilities and the highest total antioxidant capacity. This study clarifies the high antioxidant activity plant resources in Inner Mongolia grassland, and provides scientific theoretical support for the antioxidant functions, pharmaceutical and functional product development of grassland plants, and the sustainable utilization of grassland plant resources.

Key words: grassland plants, plant extracts, total phenolic, total flavonoid, total polysaccharide, total saponin, antioxidant activity